黃世佑2006-07-252018-06-282006-07-252018-06-282004-12-22http://ntur.lib.ntu.edu.tw//handle/246246/9399本研究為利用微生物發酵生產生物可分解性聚合物。使用耐鹽性細菌Haloferax mediterranei 以及Pseudomonas oleovorans ，添加各種碳源及調整其他培養基組成濃度， 以期獲致具官能基（functional groups ）之PHA （polyhydroxyalkanoates ）。 H. mediterranei 菌株在初始pH 7.0 下進行發酵時，所得菌量最高（36 小時內）乾 菌重可達5.6 g/L ;在初始pH 6.5 下進行發酵，單位菌體所累積之PHA 量為最高（Yp/x =0.09 ）。培養基中添加0.1 % KH2PO4 進行發酵時，所得菌量為最高達15.59 g/L ;但添 加0.0125 % KH2PO4 進行發酵時，所得PHA 量為最高，約0.63g/L 。 於發酵進行中，饋料各種碳源並以FT-IR 、NMR 、DSC 等儀器分析發酵產物之結 構與性質。以olive oil 及soybean oil 等碳源進行饋料，所得產物PHA 結構為PHBV ， 其PHV 所佔之比例分別為8-11 %及7-8 %，且隨著PHV 比例增加，PHBV 的熔點與玻璃轉移溫度隨之下降。P. oleovorans 菌株以sodium octanoate 當作碳源，發酵所得菌體量約3.07 g/L ，PHA 累積量約0.39 g/L ，PHA 約佔菌體乾重的13 %;在限氮條件下，菌體量約為3.04 g/L ， PHA 累積量可提升到1.09 g/L ，PHA 約佔乾菌重36 %。 當P. oleovorans 以不同碳源進行發酵時，所產生PHA 的結構也會有所不同;以 sodium octanoate 當作碳源，則產生以PHO(polyhydroxyoctanoate)為主之共聚物;當使 用undecylenic acid 為碳源時，可產生側鏈末端具有雙鍵之 PHU(Poly(3-hydroxyundec-10-enoate))結構;如使用nonanoic acid 為碳源，則可產生不同 側鏈之PHHN (Poly(3-hydroxyheptanoate-co-3-hydroxynonanoate))共聚物。 未來將使用以水/乙醇或水/乙二醇(ethylene glycol)為液態培養基之混合劑，饋各種 脂溶性碳源(如：arachidonic acid ，linolenic acid 等)，以FT-IR 、NMR 、TGA 、DSC 、等 儀器分析發酵所得PHA 之結構與性質，期能獲致特殊官能性PHA 。Biosynthesis of biodegradable polymers using halophilic bacterium Haloferax mediterranei and Pseudomonas oleovorans was conducted. Manipulations of carbon source as well as ingredients of fermentation medium were investigated. PHA (ployhydroxyalkanoate) incorporated with functional groups is anticipated. H. mediterranei grew most rapidly under pH 7.0, dry cell weight reached 5.6 g/L in 36 hrs. Accumulation of PHA in the cells reached a peak under pH 6.5 (Yp/x=0.09). Fermentation with dosage of 0.1 % KH2PO4 resulted in a high cell concentration (15.59 g/L). Under a limitation of phosphate (0.0125 % KH2PO4), high PHA content was obtained (0.63 g/L). Olive oil and soybean oil were fed during the fermentation producing PHA. The products obtained were PHBV (polyhydroxybutyrate-co-polyhydroxyvalerate) whose PHV contents were 8-11 % and 7-8 %, respectively. Tm and Tg of PHBV products obtained decreased with increase of the content of PHV. 3.07 g/L of dry cell weight were obtained using P. oleovorans and dosage of sodium octanoates. PHA content was 13 % dry weight. 3.04 g/L of dry cell weight were obtained under a limitation of nitrogen source. PHA content was 36 % dry cell weight. Different products of PHA were obtained by dosage of different carbon sources. PHOs (polyhydroxyoctanoate) were the major copolymers obtained by dosaging sodium octanoate as a carbon source. PHU [poly(3-hydroxyundec-10-enoate)] and PHHN [poly(3-hydroxyheptanoate-co-3-hydroxynonanoate)] copolymers were obtained as dosaging undecylenic acid and nonanoic acid respectively as a sole carbon source. Introducing the various functional groups into the structure of PHAs facilitates the development of synthesizing new functionality biopolymers for many uses.application/pdf524923 bytesapplication/pdfzh-TW國立臺灣大學化學工程學系暨研究所嗜鹽菌Pseudomonas oleovorans聚羥基烷酯官能基壬酸十一烯酸halophilic bacteriumployhydroxyalkanoatefunctional groupnonanoic acidundecylenic acidreporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/9399/1/922621Z002003.pdf