2019-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/704300摘要：珊瑚礁生態系涵養超過四分之一海洋生物，在生態與經濟上扮演重要的角色。日趨嚴重的海水暖化現象與因氣候變遷所產生的極端氣候，都被認為影響珊瑚礁及其生態系的功能。珊瑚是個「共生體」，隨著基因定序技術的進步，已知除了珊瑚蟲和共生藻外，還有以細菌為最大宗族群的共伴微生物，這些微生物對於維持珊瑚健康有直接相關。共伴微生物的組成會受到多種微觀與巨觀等因素影響，包括了珊瑚種類，它們在珊瑚內的位置，與珊瑚宿主所位處之環境參數。目前已知水溫增高會使珊瑚共生藻離開宿主造成珊瑚白化，進而可能造成珊瑚死亡。此外也知道珊瑚常駐的內生桿菌（Endozoicomonas）與宿主健康有關，在不健康的珊瑚中數量比健康的珊瑚少。過去探討溫度變化對珊瑚生理表現以及共伴菌族群的變動，多半是根據均溫所設的穩定水溫，或以緩慢增減溫度來進行實驗。但環境中，單日溫差變化並非穩定。對於單日溫差變化對珊瑚與共伴菌的影響，並不清楚。因此，本研究將使用不同程度的日溫差，和持續增溫的日溫差進行水缸實驗，來研究兩種台灣常見的造礁珊瑚中，微生物在黏液與組織的族群變動，尤其探討長駐型共伴菌Endozoicomonas與Acinetobacter的消長。另外，除了利用次世代定序的方式研究微生物的族群變動，也會構建珊瑚共伴菌培養體學的方法，以利未來珊瑚共伴菌的實際應用。<br> Abstract: In recent years, global warming that causes rising seawater surface temperature (SST) increasingly threaten coral reef ecosystems that provide 25% of marine organisms a home along with playing essential ecological and economic role. Corals harbor diverse microorganisms, including the symbiotic algae Symbiodinium, bacteria, viruses, algae and archaea. These microbes, together with their coral host, form an integrated holobiont, and the microbes have diverse interactions with their host and maintain coral holobiont function. Among the members in coral microbiota, bacteria are the most dominant and their population is dynamic and influenced by the host species, residences within the host, geographical difference and environmental parameters. It has been known that increasing SST causes the leaving of Symbiodinium, which may also lead the leaving of potential beneficial bacteria, such as Endozoicomonas and Acinetobacter. However, most of previous studies of thermal stress to corals and coral-associated microbes mainly relied on stable temperature or slow changing of temperature during the experimental periods, which is hard to represent the daily fluctuation of temperature in the real environments. Therefore, this project will investigate bacterial community and dynamics in coral tissue and mucus in response to the high-frequency fluctuations and the succession in the two dominant bacteria Endozoicomonas and Acinetobactera. In addition to using sequencing technology, this project will establish the method for culturomics of coral-associated bacterial strains, which will be potential biomarker for coral-health surveillance.大幅度日溫差珊瑚共生菌微生物族群變動內生桿菌不動桿菌培養體學Diurnal fluctuations of temperatureCoral associated microbesBacterial community and dynamicsEndozoicomonasAcinetobacterCulturomics