YA-YU CHIANGHaeri, SinaSinaHaeriGizewski, CarstenCarstenGizewskiStewart, Joanna D.Joanna D.StewartEhrhard, PeterPeterEhrhardShrimpton, JohnJohnShrimptonJanasek, DirkDirkJanasekWest, JonathanJonathanWest2023-09-222023-09-222013-12-0300032700https://scholars.lib.ntu.edu.tw/handle/123456789/635633This paper describes a microfluidic quenched flow platform for the investigation of ligand-mediated cell surface processes with unprecedented temporal resolution. A roll-slip behavior caused by cell-wall-fluid coupling was documented and acts to minimize the compression and shear stresses experienced by the cell. This feature enables high-velocity (100-400 mm/s) operation without impacting the integrity of the cell membrane. In addition, rotation generates localized convection paths. This cell-driven micromixing effect causes the cell to become rapidly enveloped with ligands to saturate the surface receptors. High-speed imaging of the transport of a Janus particle and fictitious domain numerical simulations were used to predict millisecond-scale biochemical switching times. Dispersion in the incubation channel was characterized by microparticle image velocimetry and minimized by using a horizontal Hele-Shaw velocity profile in combination with vertical hydrodynamic focusing to achieve highly reproducible incubation times (CV = 3.6%). Microfluidic quenched flow was used to investigate the pY1131 autophosphorylation transition in the type I insulin-like growth factor receptor (IGF-1R). This predimerized receptor undergoes autophosphorylation within 100 ms of stimulation. Beyond this demonstration, the extreme temporal resolution can be used to gain new insights into the mechanisms underpinning a tremendous variety of important cell surface events. © 2013 American Chemical Society.enjournal article10.1021/ac402881h242950192-s2.0-84889053265https://api.elsevier.com/content/abstract/scopus_id/84889053265