2018-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/662321摘要：穀胱甘&#32957; (GSH) 是植物重要的氧化還原緩衝物，並且在&#63950;醇的氧化還原信號傳遞扮演重要的角色，Glutathione reductase 負責將氧化態 GSH (GSSG) 還原成為 GSH。我們先前的研究發現弱化水稻 Glutathione reductase 1(GR1) 基因表現會嚴重抑制水稻側根之生長，顯示 GSH 的生合成與氧化還原可能&#63851;與調節水稻在正常與逆境下側根之生長，為了解其機制，目前計畫中已經成功建立 DR5 promoter::GUS 與 GR1-RNAi及 GR1-OE 雜交水稻，以了解GR1表現量降低對側根生長與auxin分布之影響；並利用 Ubiquitin promoter 建立 γ-EC synthase 持續表現，以及 Estrogen誘導表達 γ-EC synthase 的轉殖水稻，以了解持續性與誘導性大量生成 GSH 對側根發育及缺水逆境下水稻側根生長之影響。<br> Abstract: The tripeptide glutathione (GSH) is regarded as a key component in thiol redox signaling and acts as a major redox buffer against reactive oxygen species. Glutathione reductase (GR) catalyzes the reduction of glutathione disulfide into reduced GSH. Our earlier studies showed the attenuated expression of rice chloroplast/mitochondria co-localized glutathione reductase 1 (OsGR1) resulted in the inhibition of lateral root (LR) formation. Therefore, we suggested that both biosynthesis and redox regulation of GSH are important for the regulation of LR formation in rice under normal and stress conditions. To uncover the molecular mechanism, DR5 promoter::GUS x GR1-RNAi and DR5 promoter::GUS x GR1-OE rice hybrids were generated to elucidate the effects of GSH redox changes and auxin distribution on the growth and development of lateral roots. Moreover, transgenic rice harboring Ubiquitin promoter::γ-EC synthase and Estrogen-inducible promoter::γ-EC synthase were produced to reveal the effect of increased GSH levels in LR growth under normal and dehydration condition in rice.穀胱甘&#32957穀胱甘&#32957還原&#37238離層酸水稻側根GlutathioneGlutathione reductaseABARiceLateral root