JEHN-HSIAHN YANGMEI-JOU CHENMING-YIH WUChen Y.-CYU-SHIH YANGHONG-NERNG HO2020-02-132020-02-1320060015-0282https://scholars.lib.ntu.edu.tw/handle/123456789/458297Objective: To examine changes in local cytokine production in the eutopic endometrium of adenomyosis after treatment with medroxyprogesterone acetate (MPA) and danazol. Design: Prospective cohort study. Setting: Tertiary university hospital. Patient(s): Twenty-four women with adenomyosis (study group) and 22 women without adenomyosis (control group). Intervention(s): Eutopic endometrium was separated into single endometrial stromal cells (ESCs), and was cultured in vitro alone, with MPA (10-7 M), or with danazol (10-7 M) for 8 days. Main Outcome Measure(s): Messenger RNA expression of interleukin (IL)-6, IL-8, and tumor necrosis factor-α in ESCs, and their concentrations in the supernatants of ESCs. Result(s): A significant decrease of IL-6 concentration in the supernatant, measured by ELISA, was found with time of ESCs cultured with MPA and danazol in the control group, but not in adenomyosis. The IL-6 mRNA in ESCs determined by real-time quantitative polymerase chain, as well as its concentration in the supernatant, was much higher in adenomyosis than that in the control group after treatment with MPA and danazol for 8 days. Conclusion(s): Medroxyprogesterone acetate and danazol appeared to have a decreased effect on the suppression of IL-6 liberated by ESCs in adenomyosis. This aberrant secretion of IL-6 might play a role in the formation of ectopic endometrial implants in adenomyosis. ? 2006 American Society for Reproductive Medicine.[SDGs]SDG3danazol; interleukin 6; interleukin 8; medroxyprogesterone acetate; messenger RNA; tumor necrosis factor alpha; adenomyosis; adult; article; clinical article; cohort analysis; concentration (parameters); controlled study; cytokine production; drug effect; endometrium cell; enzyme linked immunosorbent assay; female; human; human cell; human tissue; in vivo culture; priority journal; prospective study; real time polymerase chain reaction; statistical significance; supernatant; university hospital; Cells, Cultured; Cohort Studies; Contraceptive Agents, Female; Danazol; Drug Combinations; Endometriosis; Endometrium; Estrogen Antagonists; Female; Gene Expression; Humans; Interleukin-6; Medroxyprogesterone 17-Acetate; Medroxyprogesterone Acetate; Stromal Cells; Uterine Diseasesjournal article10.1016/j.fertnstert.2006.04.034169898252-s2.0-33750193673