Dar-Bin ShiehChia-Hao SuFong-Yu ChangYa-Na WuWu-Chou SuJih Ru HwuJYH-HORNG CHENChen-Sheng Yeh2018-09-102018-09-102006-0809574484http://scholars.lib.ntu.edu.tw/handle/123456789/325449https://www.scopus.com/inward/record.uri?eid=2-s2.0-33748898326&doi=10.1088%2f0957-4484%2f17%2f16%2f030&partnerID=40&md5=f42b089f077c55eb332f680104c40bf4A comprehensive totally aqueous phase synthesis of nickel-nitrilotriacetate (Ni-NTA) modified superparamagnetic Fe3O4 nanoparticles is presented. The Fe3O4-NTA-Ni nanoparticles are able to perform efficient and specific purification of 6-His tagged proteins from crude cell lysates, as evidenced by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. The average binding capacity, as demonstrated by streptopain (MW 42kDa), is 0.23mg/mg (protein/Fe3O4-NTA-Ni). Considering the high affinity and specificity of the binding between hexahistidine motif and Ni-NTA, Ni-NTA modified nanoparticles could act as a module to carry 6-His tagged proteins on the particle surface with molecular orientation control, since only the 6-His domain could be attached. These modularly designed functional nanoparticles enhance cancer cell targeting, as supported by the in vitro receptor mediated targeting assay using RGD-4C-6-His fusion peptide. The nanoparticles show no significant hemolysis for human blood and could be investigated further for their in vivo functional imaging applications. ? 2006 IOP Publishing Ltd.[SDGs]SDG3[SDGs]SDG6Blood; Iron compounds; Molecular orientation; Nickel; Polypeptides; Proteins; Hemolysis; Nanoparticles; Nickel-nitrilotriacetate (Ni-NTA); Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE); Nanostructured materialsjournal article10.1088/0957-4484/17/16/0302-s2.0-33748898326WOS:000240012600030