2013-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/680867摘要：細菌性纖維素是一種由 Acetobacter xylinum所生產的天然、生物可分解聚醣。由於細菌纖維素具有特殊的奈米結構，此種生物材料已經被嘗試應用在傷口照護與組織工程方面並被稱為-永不乾燥的膜。 細菌性纖維素商品化的瓶頸在於它的生產型態。傳統的靜置培養可以產出片狀的細菌纖維 (pellicle)。此種型態的細菌纖維可以應用在許多方面，然而，其缺點在於生產效率低落。反之，許多研究團隊利用攪拌式培養來生產細菌纖維。雖然可以有效提高產率，但是此種培養方式下生產的細菌纖維是呈現不規則顆粒狀 (reticulated slurry)，此種形狀之纖維素可應用性受到很大的侷限。因此，一個可以有效率的片狀細菌纖維的生物反應器生產系統有其必要性。 纖維素的生產已經被證實和 A. xylinum的數目呈正相關。Plastic composite support (PCS) 是一個由聚丙烯以及營養成分的擠壓產物。A. xylinum 可以藉由附著在 PCS 的過程中，有效增加細胞總數。利用 PCS 生物反應器來生產細菌纖維素已經在吾人先前的研究成果中證實可行。本研究計畫將使用研究室開發之旋轉式 PCS 生物反應器，通過利用固定化細胞及探討生產條件，最佳化生產片狀細菌纖維素供修飾並應用於傷口敷料開發。亦將評估經由旋轉式PCS 生物反應器生產之細菌纖維素之材料特性以及生物可相容性。本計畫擬分三年完成。 <br> Abstract: Traditional wound dressings include cotton wool, natural or synthetic bandages and gauzes. The essential characteristic of wound dressing is to retain and create a moist environment around the wound to facilitate wound healing. Bacterially produced cellulose, which is a nature, biodegradable polysaccharide produced by Acetobacter xylinum, is being actively studied as a novel scaffold material for wound care and tissue engineering applications due to its remarkable physical properties result from the nanostructure as the never-dried membrane. The bottleneck of the commercialization of bacterial cellulose (BC) lies in the nature of cellulose morphology. Traditional static cultivation, with low productivity, can produce BC pellicle which is commonly adopted for many applications. Contrarily, BC produced under agitated cultures, though with higher productivity, is not in the pellicle form, but is produced as reticulated cellulose slurry. The applications of cellulose slurry are limited. Therefore, a robust BC production system with desired cellulose type is necessary. A novel bioreactor design that can compromise between productivity and cellulose type needs to be proposed in order to meet the increasing demand of BC in the worldwide market. High biomass density has been proved to be beneficial for BC production in many cases. Plastic composite support (PCS) is an extrusion product of a mixture of polypropylene and nutritional compounds (mainly from agricultural wastes). Biofilm grows on the PCS where microorganisms attach, and is a natural form of cell immobilization. Production of BC by using a Rotating PCS biofilm reactor (PCSRBR) has been carried out in our previous studies. BC can be produced semi-continuously without the need of reinoculation. In the present study, we would like to optimize BC production using PCSRBR reactor that can produce BC in pellicle as wound dressing materials, and, meanwhile, increase its total productivity. The produced BC will be modified by different chemicals which can enhance BC tensile strength as well as cell attachment. The modified BC will be evaluated for its materials properties and biocompatibility as a potential wound dressing biomaterial. This project will be executed in three consecutive years.醋酸菌細菌纖維素生物敷料醱酵生產生物膜反應器材料分析Acetobacter xylinumbacterial cellulosewound dressingfermentationbiofilm reactormaterials analysis