Wang A.Ren N.Shi Y.Lee D.-J.2019-05-142019-05-14200803603199https://scholars.lib.ntu.edu.tw/handle/123456789/408182Dark fermentation of microcrystalline cellulose to produce biohydrogen using mono-culture or co-culture of isolated strains was studied. A strain (X9) with high hydrogen yield from microcrystalline cellulose was isolated and identified to be closely affiliated with Clostridium acetobutylicum, ATCC 824. At 37 {ring operator} C and pH 5.0, the mono-culture of X9 yields hydrogen with a 5-h time lag and end liquid products primarily of acetate and butyrate. The co-culture of X9 with another strain, Ethanoigenens harbinenseB49, which can produce hydrogen efficiently from monosaccharides but directly from microcrystalline cellulose, produced more efficiently the biohydrogen via ethanol-type fermentation metabolism compared with mono-culture X9 test. Bioaugmentation with X9 + B49 improved cellulose hydrolysis and subsequent hydrogen production rates as compared with that of mono-culture bioaugmentation with X9. ? 2007 International Association for Hydrogen Energy.BioaugmentationBiohydrogenCo-cultureMicrocrystalline cellulose[SDGs]SDG7Cell culture; Cellulose; Hydrolysis; Bioaugmentation; Biohydrogen; Hydrogen production; Cells; Hydrogen; Hydrolysis; MicrOCRystalline Cellulosejournal article10.1016/j.ijhydene.2007.10.0172-s2.0-38849099692https://www.scopus.com/inward/record.uri?eid=2-s2.0-38849099692&doi=10.1016%2fj.ijhydene.2007.10.017&partnerID=40&md5=095dd0f6088b9e5db8ee30c66ed4457b