Tseng P.-HChiang S.-JChen S.-JCHEN-YUAN DONG2022-04-252022-04-25202116057422https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107772631&doi=10.1117%2f12.2582702&partnerID=40&md5=fcb91b88369dd6180af896ff0616d8e1https://scholars.lib.ntu.edu.tw/handle/123456789/606473In this study, the efficiency of uni- and bi-directional molecular diffusion in porcine liver tissue sections was studied. Tissue sections on the order of a few hundred microns in thickness were used. We found that bi-directional diffusion is more efficient in delivering probe molecules. Therefore, bi-directional labeling is useful in visualizing tissue architecture in three-dimensions. ? 2021 SPIE. All rights reserved.Diffusion; Enhanced labeling; Multiphoton imaging; Tissue sections; Molecules; Bi-directional; Hydrophilic and hydrophobic; Liver tissue; Molecular diffusion; Probe molecules; Three dimensions; Tissue architecture; Tissue sections; Tissueconference paper10.1117/12.25827022-s2.0-85107772631