Chen C.-F.Shiou-Hwei YehDING-SHINN CHENPEI-JER CHENJou Y.-S.2021-07-032021-07-0320051045-2257https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984578438&doi=10.1002%2fgcc.20247&partnerID=40&md5=c9d728bbfa26cf49cb5eb1b29111dc74https://scholars.lib.ntu.edu.tw/handle/123456789/568697A novel 1-cM (1.8 Mb) homozygous deletion (HD) on 13q12.11 was identified in a human hepatocellular carcinoma (HCC) cell line, SK-Hep-1, after high-density genetic marker scan and Southern blotting analysis. A loss of heterozygosity (LOH) analysis indicated that LOH frequency of the HD region in 48 pairs of HCC tissues was 52%. Interestingly, the occurrence of LOH in the 13q12.11 HD region is significantly associated with early-onset HCC, inferred from Fisher's exact test (P = 0.0047) and Mann-Whitney test (P = 0.023). Since the novel 1-cM (1.8 Mb) HD region is gene-rich with more than 37 predicted transcripts, we used a candidate gene approach by examining down-regulation of known tumor suppressor genes (TSGs), including LATS2, TG737, CRYL1, and GJB2, in HCC tissues. We detected only 14% down-regulation of the LAST2 gene that flanks the outside of the HD, in HCC tissues, by quantitative RT-PCR assays. However, we observed significant down-regulation of the TG737, CRYL1, and GJB2 genes located within the HD in 59, 64, and 71% of HCC tissues, respectively. Together, our results indicated that the identified 13q12.11 HD region contained at least three significant down-regulated TSGs, and preferential LOH in early-onset HCC patients is a putative tumor suppressor locus in HCC. ? 2005 Wiley-Liss, Inc.[SDGs]SDG3adult; aged; article; cancer cell culture; chromosome 13q; chromosome deletion; CRYL1 gene; down regulation; female; gene locus; genetic marker; GJB2 gene; heterozygosity loss; homozygosity; human; human cell; human tissue; LATS2 gene; liver cell carcinoma; male; molecular genetics; priority journal; quantitative analysis; rank sum test; reverse transcription polymerase chain reaction; Southern blotting; TG737 gene; tumor suppressor genejournal article10.1002/gcc.20247160754622-s2.0-84984578438