國立臺灣大學醫學院外科王水深2006-07-262018-07-112006-07-262018-07-112004-07-31http://ntur.lib.ntu.edu.tw//handle/246246/24524This study investigated whether a nano-meter scale of surface roughness could improve the adhesion and growth of human endothelial cells on biomaterial surface. Different molecular weights or chain lengths of polyethylene glycol (PEG) were mixed and then grafted to polyurethane (PU) surface, a model smooth surface, to form a nano-meter (nm) scale of roughness for PU-PEG surfaces (PU-PEG mix) while PEG with molecular weight of 2000 was also grafted to PU to form PU-PEG 2000 for comparison. In addition, the concept was tested on cell adhesive peptide Gly-Arg-Gly-Asp (GRGD) was photochemically grafted to PU-PEG mix and PU-PEG 2000 surfaces (e.g., PU-PEG mix -GRGD and PU-PEG 2000 -GRGD surfaces, respectively). The adhesion and growth of HUVECs for the roughness surfaces were statistical significantly better than that of smooth surface for both GRGD grafted and un-grafted surfaces, respectively. In conclusion, increased surface roughness of biomaterial surfaces even at 10~102 nm scale could enhance the adhesion and growth of HUVECs on roughness surfaces that could be worth for applications of tissue engineering.application/pdf94196 bytesapplication/pdfzh-TW國立臺灣大學醫學院外科surface roughnessnm scaleAFMHUVECsGRGDreporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/24524/1/922314B002246.pdf