2009-04-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/663894摘要：植物生育期間經常受到病原真菌、細菌等之危害，故常藉由施用化學藥劑進行病害防治；但病原菌容易產生抗藥性，需以其他替代方案協助病害問題之解決。使用天然抗微生物資材替代化學藥劑進行保護種子及苗期，即為一降低病害發生之策略。本實驗室在百合水楊酸誘導表現訊息核酸之cDNA的選殖工作中，鑑定一與植物防禦有關之解碼富含甘胺酸與半胱氨酸蛋白質的cDNA-LsGRP1；解碼基因與啟動子表現分析結果顯示LsGRP1基因具有病原與逆境誘導性。另一方面， 許多研究指出富含半胱氨酸蛋白質具有抗微生物活性在豆科植物與固氮細菌共生之報告指出，豆科植物大量表現富含甘胺酸蛋白質可限制固氮細菌的過度生長；本研究擬藉由大腸桿菌重組蛋白表現系統或是植物分子農場系統表現並回收LsGRP1蛋白，測試其抗微生物標的、活性、耐熱性、抗蛋白分解酵素能力以及植物保護功能等特性，期能有助於植物生長及種苗生產。 <br> Abstract: Chemical control is often applied for plant protection from the damage caused by bacterial and fungal pathogens. However, there are many chemical-resistant pathogens evoluted under the stress of chemical treatment. To solve the problem, it is a suitable strategy to replace chemicals with other natural antimicrobial materials in seed and seedling protection. LsGRP1 is a salicylic acid-responsive gene encoding a glycine- and cycstein-rich 138-amino acid protein present in plant extracellular matrix. Results of promoter expression assay and northern blot analysis presumed that LsGRP1 plays a role in salicylic acid and pathogen-induced defense responses in lily. It is reported that many cystein-rich proteins shows antimicrobial activities. Studies of nodulin-formation related glycine-rich protein (GRP) encoding genes indicated that highly expression of GRP in nodulin is a mechanism to limit the overgrowth of nitrogen-fixing bacteria in legume. In this study, Escherichia coli recombinant protein expression system or plant molecular farming system will be tested in production of LsGRP1 protein, and the characteristics about antimicrobial activity, antimicrobial spectrum, heat- and proteinase-resistant ability of purified LsGRP1 will be analysis to evaluate the antimicrobial function of LsGRP1. This study will provide the information of LsGRP1 protein as a plant protection agent in disease control.百合富含甘胺酸蛋白質1抗微生物胜&#32957植物保護資材LsGRP1antimicrobial peptideplant protection agent