Chen Y.-T.Yang N.-S.Lin Y.-C.Ho S.-T.Li K.-Y.Lin J.-S.Liu J.-R.MING-JU CHENJE-RUEI LIU2019-12-192019-12-19201820452322https://scholars.lib.ntu.edu.tw/handle/123456789/435674The difficulty of long-term management has produced a high rate of failure for obesity patients. Therefore, improving the efficacy of current obesity treatment is a significant goal. We hypothesized that combining a probiotic Lactobacillus Mali APS1 intervention with dieting could improve the efficacy of obesity and hepatic steatosis treatment compared to dieting alone. Mice were fed a high-fat diet for 6 weeks and then treated with: saline + normal diet and APS1 + normal diet (NDAPS1) for 3 weeks. NDAPS1 accelerated body weight loss and reduced caloric intake and fat accumulation. The fecal microbiome showed that accelerating weight loss by NDAPS1 resulted in restoring intestinal microbiota toward a pre-obese state, with alteration of specific changes in the obesity-associated bacteria. APS1 manipulated the gut microbiome's obesity-associated metabolites, followed by regulation of lipid metabolism, enhancement of energy expenditure and inhibition of appetite. The specific hepatic metabolites induced by the APS1-manipulated gut microbiome also contributed to the amelioration of hepatic steatosis. Our results highlighted a possible microbiome and metabolome that contributed to accelerating weight loss following treatment with a combination of APS1 and dieting and suggested that probiotics could serve as a potential therapy for modulating physiological function and downstream of the microbiota. ? 2018 The Author(s).[SDGs]SDG3hormone; probiotic agent; adipose tissue; animal; body weight loss; caloric intake; diet; disease model; intestine flora; Lactobacillus; liquid chromatography; mass spectrometry; metabolism; metabolomics; mouse; obesity; pathology; procedures; Adipose Tissue; Animals; Chromatography, Liquid; Diet; Disease Models, Animal; Energy Intake; Gastrointestinal Microbiome; Hormones; Lactobacillus; Mass Spectrometry; Metabolomics; Mice; Obesity; Probiotics; Weight Lossjournal article10.1038/s41598-018-23844-y2-s2.0-85045739060https://www2.scopus.com/inward/record.uri?eid=2-s2.0-85045739060&doi=10.1038%2fs41598-018-23844-y&partnerID=40&md5=89d06c7da66b062a5665acf6b7793406