Su C.-HWang B.-WDutkiewicz E.PCHENG-CHIH HSUYang Y.-L.2022-12-142022-12-14202219406029https://www.scopus.com/inward/record.uri?eid=2-s2.0-85132627182&doi=10.1007%2f978-1-0716-2349-7_3&partnerID=40&md5=316539f12826c2a9e2bf3320e2dd83fchttps://scholars.lib.ntu.edu.tw/handle/123456789/626369The surface-assisted laser desorption/ionization (SALDI) technique uses inorganic materials to aid desorption and ionization of molecules. SALDI is suitable for analyzing small molecules due to the absence of interfering signals in the low m/z range originating from the organic matrix. Imaging mass spectrometry (IMS) is a versatile imaging approach with high spatial resolution for analyzing various molecular species, but its application depends heavily on the ionization method. We have developed a functionalized titanium dioxide (TiO2) nanowire as a solid substrate for SALDI-MS detection of low-molecular-weight molecules. We apply this novel substrate for imprinting fragile specimens such as petals and further SALDI-IMS analysis. The TiO2 nanowire substrate is prepared from a commercial Ti plate by a hydrothermal process and subsequently chemically modified to improve the quality and selectivity of imprinting as well as the sensitivity of SALDI-IMS analysis. Here, the functionalized TiO2 nanowire substrate is applied to visualize the distribution of vinca alkaloids in the petal of Madagascar periwinkle (Catharanthus roseus). © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.Catharanthus roseus; Imaging mass spectrometry; Petal; Surface-assisted laser desorption/ionization; Titanium dioxide; Vinca alkaloidsVinca alkaloid; Catharanthus; diagnostic imaging; laser; matrix-assisted laser desorption-ionization mass spectrometry; procedures; Catharanthus; Diagnostic Imaging; Lasers; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Vinca Alkaloidsjournal article10.1007/978-1-0716-2349-7_3357329352-s2.0-85132627182