2011-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/661772摘要：植物生育期間經常受到病原真菌、細菌等之危害，故常藉由施用化學藥劑進行病害防治；但病原菌容易產生抗藥性，需以其他替代方案協助病害問題之解決。使用天然抗微生物資材替代化學藥劑進行保護種子、種苗與植株，即為一降低病害發生之策略。本實驗室在百合水楊酸誘導表現訊息核酸之cDNA的選殖工作中，鑑定一與植物防禦有關之解碼富含甘胺酸與半胱氨酸蛋白質的cDNA-LsGRP1；解碼基因與啟動子表現分析結果顯示LsGRP1基因具有病原與逆境誘導性。先前本研究藉由大腸桿菌重組蛋白表現系統進行LsGRP1蛋白之表現並純化，並經測試發現LsGRP1胜&#32957;片段具有廣效之抗微生物活性及抑制病害的作用。本年度擬針對十字花科病害與花卉灰黴病，分析LsGRP1之處理時間、處理部位與處理濃度對病害之防治效果，以瞭解合理施用LsGRP1的方式，並希望未來能通過發酵量產應用於植物生長及種苗生產。<br> Abstract: Chemical control is often applied in plant protection to decrease bacterial and fungal diseases. However, there are many resistant pathogens evolved under the stress of chemical treatment. To solve the problem, there is a suitable strategy to reduce chemicals with natural antimicrobial materials for seed and seedling protection. LsGRP1 is a salicylic acid-responsive gene encoding a glycine- and cycstein-rich protein present in plant extracellular matrix. In previous study, Escherichia coli recombinant protein expression system was used for production of LsGRP1 protein. It was proved that LsGRP1 peptide fragments showed antimicrobial activity against a board spectrum of fungal and bacterial pathogens. In addition, LsGRP1 showed an ability of disease suppression. In this project, the optimal timing, sites and concentration of LsGRP1 for disease control will be tested with fungal and bacterial pathogens. This study will provide a basis on the application of LsGRP1 protein as a plant protection agent against plant diseases.富含甘胺酸蛋白十字花科黑腐病菌灰黴病菌植物保護資材LsGRP1Xanthomonas campestris pv. campestrisBotrytis cinereaplant protection