SHEY-YING CHENLee, HaoHaoLeeTeng, Shih HuaShih HuaTengWang, Xiao MeiXiao MeiWangLee, Tai FenTai FenLeeHuang, Yu ChungYu ChungHuangLiao, Chun HsingChun HsingLiaoLEE-JENE TENGPO-REN HSUEH2019-11-182019-11-182018-07-0117460913https://scholars.lib.ntu.edu.tw/handle/123456789/431401© 2018 Future Medicine Ltd. Aim: We evaluated a Staphylococcus argenteus-specific diagnostic profile of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for accurate identification of this novel bacterium. Materials & methods: Staphylococcus argenteus was identified based on negative crtM gene and presence of specific sequence types. A classification model was generated by cluster analysis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer results and ClinProTools software for 25 S. argenteus and 25 methicillin-susceptible S. aureus (MSSA). The performance of the classification model was validated against 72 S. argenteus and 72 MSSA isolates. Results: With cluster analysis and classification model, differentiation of 72 S. argenteus from 72 MSSA had 100.0% accuracy by chemical extraction method and 87.5% sensitivity and 100.0% specificity by direct smear method. Conclusion: The classification model could accurately differentiate S. argenteus from MSSA.enBruker Biotyper MALDI-TOF MS | classification model | matrix-assisted laser desorption/ionization-time of flight mass spectrometry | Staphylococcus argenteus | Staphylococcus aureusBruker Biotyper MALDI-TOF MS; classification model; matrix-assisted laser desorption/ionization-time of flight mass spectrometry; Staphylococcus argenteus; Staphylococcus aureus[SDGs]SDG3adult; Article; bacterium detection; clinical classification; controlled study; diagnostic accuracy; diagnostic test accuracy study; disease classification; gene sequence; human; major clinical study; matrix assisted laser desorption ionization time of flight mass spectrometry; methicillin susceptible Staphylococcus aureus; middle aged; nonhuman; priority journal; retrospective study; sensitivity and specificity; Staphylococcus; Staphylococcus argenteus; Staphylococcus argenteus infection; Staphylococcus infection; chemistry; classification; matrix-assisted laser desorption-ionization mass spectrometry; microbiological examination; procedures; Staphylococcus; validation study; Bacteriological Techniques; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staphylococcusjournal article10.2217/fmb-2018-0015299522402-s2.0-85051393512WOS:000444865600005https://api.elsevier.com/content/abstract/scopus_id/85051393512