Tsai I.-L.Liu H.-Y.PING-HUNG KUOJANN-YUAN WANGLI-JIUAN SHENCHING-HUA KUO2020-08-132020-08-1320111618-2642https://www.scopus.com/inward/record.uri?eid=2-s2.0-80255135492&doi=10.1007%2fs00216-011-5285-8&partnerID=40&md5=95c019fce73a54e4a2b2f8a8a5c7af17https://scholars.lib.ntu.edu.tw/handle/123456789/512492Tuberculosis (TB), an infectious disease caused by Mycobacterium tuberculosis, infects approximately one third of the current world population. Isoniazid is one of the most frequently used first-line anti-TB drugs. In this study, we developed a sensitive cation-selective exhaustive injection-sweeping- micellar electrokinetic chromatography method (CSEI-Sweep-MEKC) for analyzing isoniazid in human plasma. Parameters including acetonitrile (ACN) percentage in the separation buffer; the injection time, and concentration of the high-conductivity buffer; sodium dodecyl sulfate (SDS) concentration; phosphate concentration in the sample matrix; and the sample injection time were all optimized to obtain the best analytical performance. The optimal background electrolyte comprised 50 mM phosphate buffer, 100 mM SDS, and 15% ACN. Non-micelle background electrolyte, containing 75 mM phosphate buffer and 15% ACN, was first injected into the capillary, followed by a short plug of 200 mM phosphate (high-conductivity buffer). Run-to-run repeatability (n?=?3) and intermediate precision (n?=?3) of peak area ratios were found to be lower than 8.7% and 11.4% RSD, respectively. The accuracy of the method was within 98.1-106.9%. The limit of detection of isoniazod in human plasma was 9 ng mL -1. Compared with conventional MEKC, the enhancement factor of the CSEI-Sweep-MEKC method was 85 in plasma samples. The developed method was successfully used to determine isoniazid concentration in patient plasma. The results demonstrated that CSEI-Sweep-MEKC has the potential to analyze isoniazid in human plasma for therapeutic drug monitoring and clinical research. ? 2011 Springer-Verlag.[SDGs]SDG3Analytical performance; Back ground electrolyte; Biological samples; Cation-selective exhaustive injection; Clinical research; Electrokinetic chromatography; Enhancement factor; Human plasmas; Infectious disease; Injection time; Isoniazid; Limit of detection; Micellar electrokinetic chromatography; Mycobacterium tuberculosis; Peak area ratios; Phosphate buffers; Phosphate concentration; Plasma samples; Quantitative determinations; Run to run; Sample injection; Sample matrix; Separation buffers; Sodium dodecyl sulfate; Sweeping; Therapeutic drug monitoring; World population; Acetonitrile; Concentration (process); Electrodynamics; Electrolytes; Liquid chromatography; Optimization; Patient monitoring; Plasma (human); Plasmas; Positive ions; Sodium sulfate; Hydrazine; acetonitrile; acetonitrile derivative; buffer; cation; dodecyl sulfate sodium; isoniazid; tuberculostatic agent; article; chemistry; human; metabolism; micellar electrokinetic chromatography; micelle; Acetonitriles; Antitubercular Agents; Buffers; Cations; Chromatography, Micellar Electrokinetic Capillary; Humans; Isoniazid; Micelles; Sodium Dodecyl Sulfate; Mycobacterium tuberculosisjournal article10.1007/s00216-011-5285-8218336342-s2.0-80255135492