Wu, Yi Hsieng SamuelYi Hsieng SamuelWuLin, Yi LingYi LingLinCHIEN HUANGCHIH-HSIEN CHIUNakthong, SasitornSasitornNakthongYI-CHEN CHEN2023-04-172023-04-172020-04-0100225142https://scholars.lib.ntu.edu.tw/handle/123456789/630235https://www.scopus.com/record/display.uri?eid=2-s2.0-85079362202&origin=resultslistBACKGROUND: Cardio-renal syndrome (CRS) is an integrative problem related to chronic malnutrition, obesity, etc. Amino acids and peptides are regarded as protective and essential for tissues. Pepsin-digested chicken liver hydrolysates (CLHs), which are made from the byproducts of the poultry industry, are amino-acid based and of animal origin, and may be protective against the myocardial and renal damage induced by a high-fat diet (HFD). RESULTS: Our results showed that CLHs contain large quantities of anserine, taurine, and branched-chain amino acids (BCAAs), and supplementing the diet with CLHs reduced (P < 0.05) weight gain, liver weight, peri-renal fat mass / adipocyte-area sizes, serum total cholesterol (TC), aspartate aminotransferase (AST), and low-density lipoprotein cholesterol (LDLC) levels in HFD-fed mice but increased (P < 0.05) serum high-density lipoprotein cholesterol (HDLC) levels. By histological analyses, CLHs alleviated (P < 0.05) renal lipid deposition and fibrosis, as well as cardiac fibrosis and inflammation of HFD-fed mice. Meanwhile, increased (P < 0.05) inflammatory and fibrotic cytokines levels in the myocardia of the HFD-fed mice were downregulated (P < 0.05) by CLH supplementation. Regarding autophagy-related protein levels, protective effects of CLHs on the myocardia against HFD feeding may result from the early blockade of the autophagy pathway to prevent autophagosome accumulation. CONCLUSION: Functional CLHs could be a novel food ingredient as a cardio-renal protective agent against a high-fat dietary habit in a niche market. © 2020 Society of Chemical Industry.enautophagycardio-renal damagechicken liver hydrolysatehigh-fat dietmyocardium[SDGs]SDG2[SDGs]SDG3aspartate aminotransferase; cholesterol; protein hydrolysate; adverse event; animal; autophagy; blood; C57BL mouse; cardiac muscle; cardiorenal syndrome; chemistry; chicken; fibrosis; kidney; lipid diet; liver; male; pathology; Animals; Aspartate Aminotransferases; Autophagy; Cardio-Renal Syndrome; Chickens; Cholesterol; Diet, High-Fat; Fibrosis; Kidney; Liver; Male; Mice, Inbred C57BL; Myocardium; Protein Hydrolysatesjournal article10.1002/jsfa.10261319510162-s2.0-85079362202WOS:000512368200001https://api.elsevier.com/content/abstract/scopus_id/85079362202