Wang Y.-T.PING-HUEI TSENGCHI-LING CHENDER-SHENG HANChi Y.-C.FEN-YU TSENGWEI-SHIUNG YANG2020-03-312020-03-3120171475-2840https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018486306&doi=10.1186%2fs12933-017-0522-x&partnerID=40&md5=03e746c780a82f83be664cfb0922474fhttps://scholars.lib.ntu.edu.tw/handle/123456789/481837Background: Ribonuclease-L (RNase-L) was known to be a ubiquitous enzyme involved in several cellular functions, especially innate immunity. It was recently shown to participate in adipogenesis in rodents. Here, we developed a method to measure serum levels of RNase-L and analyzed the relationship between RNase-L and metabolic syndrome (MetS). Methods: A total of 396 subjects were recruited from a health check-up program. An in-house RNase-L immunoassay was developed. The serum RNase-L levels of these subjects were measured, and the association of MetS-related factors with RNase-L levels was assessed. Results: The mean serum level of RNase-L of the subjects with MetS were lower than those without (16.5 ± 6.4 vs. 18.4 ± 8.0 μg/ml, P = 0.018). The subjects with central obesity, elevated blood pressure, or impaired fasting glucose also had lower serum RNase-L levels in comparison to those without. In multivariate linear regression analysis, diastolic blood pressure (β = -0.129, P = 0.024) and high-density lipoprotein cholesterol (HDL-C) (β = 0.127, P = 0.036) were related to serum RNase-L. For every 5 μg/ml increase in serum RNase-L levels, it is associated with a reduced risk of MetS (OR 0.83, 95% CI 0.71-0.98, P = 0.028), central obesity (OR 0.82, 95% CI 0.71-0.94, P = 0.005), or low HDL-C (OR 0.86, 95% CI 0.74-1.00, P = 0.042). Moreover, age is inversely related to serum RNase-L levels in various analyses. Conclusions: The serum RNase-L levels were inversely associated with MetS, unfavorable metabolic profiles, and age. ? 2017 The Author(s).[SDGs]SDG3antidiabetic agent; antihypertensive agent; antilipemic agent; glucose; high density lipoprotein cholesterol; ribonuclease L; 2-5A-dependent ribonuclease; biological marker; ribonuclease; adult; age; Article; blood analysis; cholesterol blood level; controlled study; diabetes mellitus; diastolic blood pressure; disease association; female; human; hyperlipidemia; hypertension; immunoassay; impaired glucose tolerance; major clinical study; male; medical examination; metabolic syndrome X; middle aged; obesity; risk reduction; aging; anthropometry; blood; enzyme linked immunosorbent assay; metabolic syndrome X; Obesity, Abdominal; pathology; procedures; Adult; Aging; Anthropometry; Biomarkers; Endoribonucleases; Enzyme-Linked Immunosorbent Assay; Female; Humans; Male; Metabolic Syndrome X; Middle Aged; Obesity, Abdominaljournal article10.1186/s12933-017-0522-x283999252-s2.0-85018486306