Tsai C.-W.CHIANG I-NIJYH-HORNG WANGTai-Horng Young2020-02-102020-02-1020182169-1401https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032675765&doi=10.1080%2f21691401.2017.1394873&partnerID=40&md5=e5a7faaa70160570e58e0e4a9df684dbhttps://scholars.lib.ntu.edu.tw/handle/123456789/455871This study evaluated the effect of chitosan, poly vinyl alcohol (PVA) and poly (2-hydroxyethyl methacrylate) (pHEMA) on delaying the human fibroblast senescence. Cells could form suspending multicellular spheroids on these biomaterials, but only chitosan was capable of decreasing the SA β-gal activity and increasing the proliferation ability of senescent fibroblasts. Therefore, in addition to the structure of multicellular spheroids, chitosan itself should play an important role in delaying fibroblast senescence. The main difference of senescence-related protein expressions for cells cultured on chitosan, PVA and pHEMA occurred on the TGF-β signaling pathway. In addition to the intracellular TGF-β expression, the extracellular TGF-β expression was also downregulated. Chitosan with cationic amino structure was assumed to bind with anionic TGF-β by forming polyelectrolyte complexes. This assumption was demonstrated by directly adding chitosan into the medium to downregulate the cell TGF-β expression and further to delay cell senescence, indicating TGF-β signaling pathway was involved in the chitosan-mediating fibroblast senescence process. Finally, the delaying cell senescence ability of chitosan increased with increasing the amount of amino groups in chitosan and its ionization degree. In summary, these results provide important information for considering the application of chitosan in the future cell therapy and regeneration medicine. ? 2017, ? 2017 Informa UK Limited, trading as Taylor & Francis Group.[SDGs]SDG3Cell culture; Chitosan; Enzyme activity; Fibroblasts; PHEMA; Polyelectrolytes; Signaling; Multicellular spheroid; Poly(2-hydroxyethyl methacrylate); Polyelectrolyte complexes; Protein expressions; Regeneration medicine; senescence; Signaling pathways; spheroids; Cell signaling; chitosan; ethidium; homodimer; polyelectrolyte; polymacon; polyvinyl alcohol; protein p16; protein p53; transforming growth factor beta; chitosan; polymacon; polyvinyl alcohol; transforming growth factor beta; Article; cell aging; cell culture; cell density; cell proliferation; cell proliferation assay; controlled study; down regulation; fibroblast; human; human cell; ionization; multicellular spheroid; nuclear magnetic resonance spectroscopy; protein expression; TGF beta signaling; Western blotting; animal; biosynthesis; cell aging; cytology; down regulation; drug effect; metabolism; signal transduction; Animals; Cellular Senescence; Chitosan; Down-Regulation; Fibroblasts; Humans; Polyhydroxyethyl Methacrylate; Polyvinyl Alcohol; Signal Transduction; Transforming Growth Factor betajournal article10.1080/21691401.2017.1394873290812442-s2.0-85032675765