Chen, Jr WeiJr WeiChenLin, Yi LingYi LingLinCHUNG-HSI CHOUWu, Yi Hsieng SamuelYi Hsieng SamuelWuSHENG-YAO WANGYI-CHEN CHEN2023-04-182023-04-182020-03-0117564646https://www.scopus.com/record/display.uri?eid=2-s2.0-85078580970&origin=recordpagehttps://scholars.lib.ntu.edu.tw/handle/123456789/630244A high-fat diet is a major risk factor for obesity and heart failure. Our previous study has demonstrated that protease A-digested crude-chalaza hydrolysates (CCH-As) with a specific free amino-acid and carnosine/anserine profile show lipid-lowering and antioxidant activities against chronic alcohol consumption. The antiobesity and hypolipidemic effects of our CCH-As against a high-fat diet was to investigated in this study. In high-fat-diet fed hamsters, supplementing CCH-As reduced liver/perirenal-adipose-tissue sizes, and serum triglyceride, LDLC/HDLC, and TBARS values (p < 0.05). Supplementing CCH-As increased lipolysis (hormone sensitive lipase, carnitine palmitoyltransferase 1, and uncoupling protein 2) in the perirenal-adipose tissues of high-fat-diet fed hamsters (p < 0.05); meanwhile decreased cholesterol biosynthesis (squalene synthase), and upregulated bile-acid biosynthesis (cholesterol 7 alpha-hydroxylase) and cholesterol clearance ability (low-density lipoprotein receptor) in livers (p < 0.05). Daily fecal lipid and bile-acid outputs were also increased in high-fat-diet fed hamsters cotreated with CCH-As. Therefore, this CCH-A can be characterized as an antiobesity and cardioprotective ingredient.Crude chalaza hydrolysates; Fecal lipid/bile-acid output; Lipid metabolism; Obesity; Serum antioxidant level; Serum lipid[SDGs]SDG3journal article10.1016/j.jff.2020.1037882-s2.0-85078580970WOS:000518708200006https://api.elsevier.com/content/abstract/scopus_id/85078580970