ANDY SUNJEAN-SAN CHIAWON-BO WANGCHUN-PIN CHIANG2019-09-022019-09-0220050192-415Xhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-23944504537&doi=10.1142%2fS0192415X05003168&partnerID=40&md5=815d9392bbf1689a5d426c0666a33efbhttps://scholars.lib.ntu.edu.tw/handle/123456789/417098Recurrent aphthous ulcerations (RAU) represent a common oral mucosal disease with altered humoral and cellular immunities. Tien-Hsien liquid (THL) is an extract of Chinese medicinal herbs with immunomodulating effects. Our previous study found that THL can modulate the antigen-stimulated proliferative response of peripheral blood mononuclear cells and T-cells isolated from RAU patients. In this study, we further tested whether THL can modulate the antigen-stimulated cytokine production by T-cells isolated from RAU patients. To achieve this goal, T-cells isolated from 19 RAU patients were incubated with phytohemagglutinin (PHA), glutaraldehyde-inactivated tetanus toxoid (TT), glucosyltransferase D (GtfD), or antigens of Streptococcus mutans in the presence or absence of THL. The levels of interleukin (IL)-2, interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), IL-6, or IL-10 in the supernatants of T-cell cultures were measured by cytokine enzyme-linked immunosorbent assay (ELISA) kits. We found that THL significantly increased the PHA- or TT-stimulated TNF-α, IL-6, and IL-10 production by T-cells isolated from RAU patients. However, THL could also significantly decrease the TT-stimulated IL-2 production, the GtfD-stimulated IL-2, TNF-α, IL-6 and IL-10 production, and the S. mutans-stimulated IFN-γ, TNF-α, and IL-10 production by T-cells isolated from RAU patients. These results indicate that THL can modulate the antigen-stimulated cytokine production by T-cells isolated from RAU patients. Because RAU is probably a Thl-mediated disease with elevated levels of IL-2, IFN-γ, TNF-α and IL-6 in either the patient's sera or oral lesions and these increased levels of cytokines can be reduced by THL, we suggest that THL may be a potential immunoceutical agent for treatment of RAU. ? 2005 World Scientific Publishing Company Institute for Advanced Research in Asian Science and Medicine.en-US[SDGs]SDG3astragalus extract; Atractylodes extract; Chinese drug; Clematidis extract; Cordyceps sinensis extract; gamma interferon; ginseng extract; glucosyltransferase; glutaraldehyde; Glycyrrhiza extract; herbaceous agent; Indigo pulvereta extract; interleukin 10; interleukin 2; interleukin 6; Ligustrum lucidum extract; Margarita extract; Oldenlandia diffusae extract; phytohemagglutinin; Pogostemon cablin extract; Polyporus umbellatus extract; solanum nigrum extract; tetanus toxoid; tien hsien liquid; Trichosanthes extract; unclassified drug; adult; aphthous ulcer; article; Astragalus plant; Chinese herb; Chinese medicine; Clematidis; clinical article; controlled study; culture medium; cytokine production; drug mechanism; enzyme linked immunosorbent assay; female; human; immunomodulation; immunostimulation; Indigo pulvereta; male; Margarita; Oldenlandia; Oldenlandia diffusae; peripheral blood mononuclear cell; plant root; pogostemon cablin; Polyporus; polyporus umbellatus; recurrent disease; Solanum nigrum; Streptococcus mutans; T lymphocyte; Th1 cell; Adult; Antigens, Bacterial; Cytokines; Drugs, Chinese Herbal; Female; Glucosyltransferases; Humans; Male; Middle Aged; Phytohemagglutinins; Recurrence; Stomatitis, Aphthous; Streptococcus mutans; T-Lymphocytes; Tetanus Toxincorrigendum10.1142/S0192415X05003168161735302-s2.0-23944504537