Tsai C.-P.Chen C.-Y.Hung Y.FU-HSIUNG CHANGMou C.-Y.2020-01-212020-01-21200909599428https://www.scopus.com/inward/record.uri?eid=2-s2.0-68349141561&doi=10.1039%2fb905158a&partnerID=40&md5=a829bd2156a46aadfcd8f5a00a45010dhttps://scholars.lib.ntu.edu.tw/handle/123456789/452193In this work, we conjugate anti-HER2/neu mAb (monoclonal antibody) to green fluorescent dye loaded mesoporous silica nanoparticles (Her-Dye@MSN) through a polyethylene glycol (MW = 5000) spacer. We examine their targeting properties toward HER2/neu over-expressing breast cancer cells and their internalization into the cells. Her-Dye@MSN nanoparticles exhibit a high targeting efficiency and the specific targeting capability is strongly affected by the mAb density on the Her-Dye@MSN. The time-course of competitive experiments with free antibody indicates that Her-Dye@MSN serves as a multivalent ligand. Moreover, Her-Dye@MSNs are internalized into cells through a receptor-mediated endocytosis and may escape from endosome to cytosol. The results reported here further support the potential of MSNs as a multifunctional smart nanocarriers for cell imaging and drug delivery. ? 2009 The Royal Society of Chemistry.[SDGs]SDG3Breast cancer cells; Cell imaging; Competitive experiments; Cytosols; Endosomes; Fluorescent dyes; Functionalized; Mesoporous silica nanoparticles; Multivalent ligands; Nanocarriers; Receptor-mediated endocytosis; Targeting capability; Drug delivery; Mesoporous materials; Monoclonal antibodies; Nanoparticles; Polyethylene glycols; Polyethylene oxides; Silica; Surface plasmon resonance; Cell membranesjournal article10.1039/b905158a2-s2.0-68349141561