職業醫學與工業衛生研究所;Institute of Occupational Medicine and Industrial HygieneLUO, JIIN-CHYUANJIIN-CHYUANLUOCHENG, TSUN-JENTSUN-JENCHENG2008-07-102018-06-292008-07-102018-06-292003http://ntur.lib.ntu.edu.tw//handle/246246/80252Aims: To determine the presence of Asp13-p21-ki-ras oncoprotein and p53 oncoprotein in the plasma of vinyl chloride monomer (VCM)-workers in Taiwan. Methods: We used enhanced chemiluminescence (ECL) western blotting to detect Asp13-p21-ki-ras and ELISA to detect mutant p53 protein (p53 -Ag) and anti-p53 antibodies (p53-Ab) in the plasma of VCM- exposed workers. Results: Twenty-five out of 251 (10%) VCM- workers were positive for Asp 13-p21-ki-ras in plasma, but 0 out of 36 controls were positive. There were 15 out of 95 ( 15.8%) plasma-positives among the more highly exposed (> 480 ppm-month) workers and 10 out of 156 (6.4%) plasma- positives among the lesser exposed (less than or equal to480 ppm- month). Compared to the unexposed controls, age and drinking adjusted odds ratios (95% Cl) were 1.2 (0.1, 9.8) in the lower exposed workers, and 4.8 (0.8, 28) in the higher exposed workers, and there was a significant linear trend between exposure and plasma positivity (P = 0.001). Thirty- three out of 251 (13.2%) VCM-workers were positive for the p 53 over-expression (10% with positive p53-Ag and 2 .8% with positive p53-Ab). There was a significant association between cumulative VCM exposure concentration and positive p 53 expression (P = 0.032) among VCM-workers after adjusting for age, hepatitis, drinking and smoking status. Conclusions: Asp I 3-p21-ki-ras oncoprotein and p53 over-expression (p 53-Ag or p53-Ab) can be found in the plasma of VCM-workers in Taiwan, and a significant dose- response relationship exists between plasma oncoproteins expression and VCM exposure. (C) 2003 International Society for Preventive Oncology. Published by Elsevier Science Ltd. All rights reserved.application/en-USP53 PROTEINCHEMICAL CARCINOGENESISSERUM ONCOPROTEINSLUNG- CANCERMUTATIONSEXPRESSION[SDGs]SDG3journal article