2004-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/704863摘要：柑桔破葉病(tatter leaf)與萎縮病、黃龍病並稱是亞洲最嚴重的三大柑桔系統性病害。該病是由柑桔破葉病毒（Citrus tatter leaf virus = CTLV）所引起，分類上隸屬於Capillovirus屬，基因體為(+)ssRNA，長度650nm。此病毒引起枳橙類柑桔產生破葉病徵，並在許多柑桔品種上造成砧木與接穗不親和的裂痕，影響植株發育且易受強風摧折。全世界對此重要病害的研究尚屬不多，申請人多年來一直致力於柑桔病毒與類病毒病害之研究，已累積不少學術成果。CTLV是我國柑桔防疫的要項之一，本研究計畫之施行將有助於整體柑桔防疫政策之達成。此計畫擬從生物性與分子性兩方面探討並鑑定不同的CTLV系統，並且同時研發各種快速偵測法，包括RT-PCR、單元抗體、Real-time RT-PCR等相關偵測技術，以供防疫檢疫之應用。因為CTLV在柑桔寄主體內濃度低，純化十分耗時；再者，其病毒系統可能相當複雜，加上柑桔品種多，植株生長緩慢，故柑桔病毒的生物性或生態性試驗常需較長時間，一般皆擬定三年以上的試驗流程，以取得較客觀準確的成果。基於此因，本計畫擬進行三年連續性之研究，以達成所有<br> Abstract: Citrus tatter leaf, tristeza and huanglongbing are the most important systemic diseases of citrus. Citrus tatter leaf virus (CTLV), the pathogen causing tatter leaf, are categorized into the genus Capillovirus consisting of a single-stranded positive-sense RNA genome packaged in 650nm flexuous particles. CTLV induced the “tatter leaf” symptom in citrange hosts and “bud-scion crease” in many other citrus cultivars, which retarded citrus growth and sometimes broke at the bud union when strong wind blew. CTLV is a main subject in the policy of integrated pest control of citrus in Taiwan. This research project is dedicated to categorize the different CTLV strains from the biological and molecular level, also develop rapid detection techniques for CTLV with several methods such as RT-PCR, monoclonal antibodies and Real-time RT-PCR. Strain-categorization studies are helpful for understanding the variations and pathogenicities of CTLV in different citrus cultivars from various cultivated areas. Designing an experiment for citrus diseases requires long-term data gathering to obtain objective biological data. Thus, a three-year continuous trial is proposed. The collection and selection of CTLV isolates, bioassays with graft-inoculation tests, development of a rapid RT-PCR assay for CTLV detection, and full genomic sequencing of several representative CTLV isolates will be carried out during the first year. The second year will focus on the preparation of monoclonal antibodies against CTLV, development of quantitative monitoring of CTLV by Real-time RT-PCR techniques, and further collections of foreign and domestic special CTLV isolates. During the last year, we expect to complete the categorization of CTLV strains, determine the phylogenetic relationships among the strains, develop the RT-PCR assay for specific detections of CTLV strains, survey the distribution of CTLV strains in Taiwan, and select good rootstock-scion combinations resistant (tolerant) to CTLV infection according to the results of inoculation tests.柑桔破葉病毒系統鑑別快速偵測Citrus tatter leaf virusstrain-identificationrapid detection