臺大醫院;臺大醫院-內科部;Tsai, Kun-HsiKun-HsiTsaiLee, Nien-HungNien-HungLeeChen, Guei-YingGuei-YingChenHu, Wei-SyunWei-SyunHuTsai, Chen-YenChen-YenTsaiChang, Mu-HsinMu-HsinChangJong, Gwo-PingGwo-PingJongKuo, Chia-HuaChia-HuaKuoTzang, Bor-ShowBor-ShowTzangTsai, Fuu-JenFuu-JenTsaiTsai, Chang-HaiChang-HaiTsaiHuang, Chih-YangChih-YangHuang2014-02-142018-07-112014-02-142018-07-112013http://ntur.lib.ntu.edu.tw//handle/246246/259151Previous studies from our lab showed that increase in AngIl in H9c2 cells causes elevated IGFII and IGFIIR through MEK and JNK, leading to rise in intracellular calcium, calcineurin activation by PLC-beta 3 via G alpha q, insertion into mitochondrial membranes of Bad, and apoptosis via caspases 9 and 3. Codonopsis pilosula is traditionally used to lower blood pressure. The purpose of our study is to investigate if C. pilosula attenuates AngII plus Leu(27)-IGFII-induced calcium influx and apoptosis in H9c2 cardiomyoblasts. C pilosula significantly attenuated AngII induced IGFIIR promoter activity. Leu(27)-IGFII was applied to enhance the Angll effect. C pilosula also reversed Ca2+ influx, MOMP and apoptosis increased by AngIl plus Leu(27)-IGFIL Molecular markers in IGFIIR apoptotic pathway (IGFIIR, calcineurin, etc.) and IGFIIR-G alpha q association were downregulated by C pilosula. However, p-Bad(Ser136) and BcI-2 were increased. Therefore, C pilosula suppresses Angll plus Leu(27)-IGFII-induced IGFII/IGFIIR pathway in myocardial cells. (C) 2012 Elsevier Ltd. All rights reserved.112 bytestext/htmlAngiotensin IIApoptosisCalcium influxCodonopsis pilosulaLeucine(27)-insulin like growth factor IIMitochondrial outer-membrane permeability[SDGs]SDG3Angiotensin II; Apoptotic pathways; Calcineurin; Calcium influx; Caspases; Codonopsis pilosula; H9c2 cells; Insulin like growth factor II; Insulin-like growth factor; Intracellular calcium; Mitochondrial membranes; Molecular marker; Myocardial cells; Promoter activities; Amino acids; Blood pressure; Calcium; Cell membranes; Mitochondria; Cell death; angiotensin II; apoptotic protease activating factor 1; calcineurin; calcium ion; Codonopsis pilosula extract; cytochrome c; leucine; protein BAD; somatomedin B receptor; animal cell; apoptosis; article; calcium cell level; calcium transport; cell membrane permeability; Chinese medicine; Codonopsis pilosula; controlled study; cytosol; down regulation; heart muscle cell; mitochondrial membrane; mitochondrial membrane potential; nonhuman; protein phosphorylation; rat; signal transduction; Apoptosis; Calcium; Cell Line; Codonopsis; Gene Expression; Humans; Myocytes, Cardiac; Receptor, IGF Type 2; Signal Transduction; Codonopsis pilosula10.1016/j.foodchem.2012.11.056http://ntur.lib.ntu.edu.tw/bitstream/246246/259151/1/index.html