施文彬臺灣大學:機械工程學研究所陳資憲Chen, Tz-ShianTz-ShianChen2010-06-302018-06-282010-06-302018-06-282009U0001-1008200913282900http://ntur.lib.ntu.edu.tw//handle/246246/187210本研究設計一具有全血分離功能之生醫晶片，藉由嵌入在微流道中的濾層，將血球與血漿做分離。製作研發多孔隙的尼龍材料作為濾層，此濾層孔徑大小約100 nm。血漿中的特殊抗原可以通過此濾層之孔洞。可嵌入濾層的微流道利用毛細力幫助血漿在其內流動。此晶片具有可拋棄的特性，並可運用於重點照護檢驗( point-of-care diagnostics)或微全分析系統 (micro total analysis system)。並且從表面能量理論出發，對在毛細管內的流動的液體建立模型與分析。最後建立一個觀測測量實驗，量測毛細管內新月形液面的變化。藉由所拍攝的新月形液面形狀，分析新月形液面的改變，並將實驗結果與理論模型做比較。A bio-chip with the capability of conducting blood separation for whole blood diagnostics has been proposed in this study. Blood cells and serum are separated by embedding filters in the microchannel. The filter have been fabricated and evaluated. The filter is made of porous nylon with the pore size of about 100 nm. Specific antibodies in the serum can pass through the filter. The microchannel which contains the filter has been properly designed so that the serum flow is facilitated by the capillary force. The fabricated device is disposable and can be potentially applied in point-of-care diagnostics or micro total analysis system (μTAS). And base on the surface energy theory to model and analyze the liquid flow in a capillary. Final, an observation experiment of measuring the variation of meniscus in a capillary is set up. By capturing the figures of meniscus shape, analyze the meniscus variation and compare the experiment data with the model solution.Table of Contents 謝 I要 IIbstract IIIable of Contents IVist of Figures VIIIist of Tables XIIIist of Appendix Figures XIIIhapter1. Introduction 1.1. Background 1.2. Motivation 2.3. Document Review 3hapter2. Design Principle and Consideration 13.1. Design Concept and Purpose 13.2. Buckingham Pi Theorem 14.2.1. Bond Number 14.2.2. Capillary Length 16.2.3. Capillary Number 17.2.4. Theory Analysis 17.3. Capillarity 18.3.1. Washburn’s Law 18.3.2. Young-Laplace Equation 19.3.3. Surface Energy 20.4. Darcy’s Law for Porous Material 22.5. Blood Properties 23.5.1. Blood Composition 23.5.2. Blood Cells 23.5.3. Blood Flow Characteristic 24.5.4. Blood Diagnostics 25hapter3. Blood Separation Chip 26.1. Material 26.2. Blood Separation Chip Fabrication 27.2.1. Porous Nylon Filter 27.2.2. Poly-di-methyl-siloxane (PDMS) Microchannel 34.2.3. Poly-methyl-meth-acrylate (PMMA) Mold 37.2.4. Silicon Substrate and Oxygen Plasma Bonding 38.3. Whole Blood Filtration Experiment 38.4. Summary 40hapter4. Capillary Modeling and Observation of Meniscus Evolution 41.1. Capillary Modeling 41.2. Observation of Meniscus Evolution 56.2.1. Capillary Channel Chip Fabrication 56.2.2. Observation Apparatus Setting 60hapter5. Experiment Results and Discussion 63.1. Observation Experiment 63.1.1. Meniscus Evolution 63.1.2. Relation between Pressure Drop and Meniscus 69.2. Measurement Method 73.3. Experiment Result 73.4. Discussion 77hapter6. Conclusion and Future Work 79.1. Blood Separation Chip 79.2. Capillary Modeling 79.3. Future Work 80ppendix: a 3D Biological Filtration Matrix using Self-assembled Microspheres inside Microchannels and Gelatin Sacrificial layer 82bstract 82ntroduction 83esign and Fabrication 83emonstration and Experiment Result 91onclusion 93eference 96en-US多孔隙分離毛細管表面能量新月形液面壓力阻礙porousseparationcapillarysurface energymeniscuspressure barrierthesis