2006-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/663147摘要：一、種子細菌性病害檢測技術之研發(中興大學曾國欽) 本研究擬針對重要之種媒細菌開發快速檢測之標準流程，本研究將利用前期所 開發之十字花科黑腐病菌檢測技術進行田間實際檢測，並對相關影響因子進行探 討，本研究所開發出之十字花科黑腐病菌檢測標準流程將提供防檢疫相關人員檢 測十字花科黑腐病菌之用。 二、開發種苗及其使用資材媒介真菌之檢疫技術(中興大學陳隆鐘) 本計畫擬針對種苗及使用資材來作檢測，應用分子標記技術進行種子品種的純 度鑑定，並同時進行種子健康分子檢測的工作，以核酸定量系統為平台，結合基 因資料庫，並配合各種病害發病門檻的資料，將可在短期內預測病害發生或進展 的可能性，進而可應用於檢防疫工作，以期使此檢測技術符合國際規範標準作業 程序之訂定，並得到國際標準之認證，期與國際間各國檢疫接軌。此外，高靈敏 度技術亦可用於特定核酸序列的定量追蹤研究上，以量化資料取代電泳條帶分析 ，或是開發為商品化的檢測套組供政府相關單位及蔬菜種苗業者使用。 三、Xanthomonas屬檢疫病原細菌檢測之研發(輔仁大學李永安) 本計畫目標在於針對植物檢疫有害生物名單之Xan<br> Abstract: 1. Development of detection techniques for bacterial seed-borne diseases The object of this study is development of rapid detection techniques for seed transmission bacteria. In this study, we will test the diseased samples and natural infected seed from the field by the rapid detection technique of Xanthomonas campestris pv. campestris. The effected factors of detection of X. campestris pv. campestris will be discussed in this study, too. The standard operating procedures (SOPs) of detection of X. campestris pv. campestris developed in this study will help for detection of X. campestris pv. campestris. 2. Development of quarantine techniques for seedling and the material of agriculture production In order to construct a plant form which is design to screen the seedling and the material of agriculture production, the identification with quality of seedling species will be supported by molecule health supervision and the plant form of nuclear acid qualification system. Further more, the connection of data side servicing base and the line transporting of disease event report will be used to predict and assess to realize the process of disease and the possibility of happening immediately. Therefore, the technology which is built up will be adapted to address the work of quarantine and to pass the ISO of national community. Besides, super sensitive tech of detecting the sequence will be allowed to be used to pursuit the information and evident of research. On the other hand, by exchange quantified data from PAGE and the developing the commercial good for those who generate the vegetable and seeding will be a good looking in the future. 3. Development of detection methods of plant pathogenic bacteria of Xanthomonas The objective of this plan is to develop seed detection and pathogen identification methods for plant pathogenic bacteria of Xanthomonas in the plant quarantine pest list. The plan will develop selective and differential media, and molecular detection and identification methods which can differentiate the species and pathovars of Xanthomonas. These methods will be presented as standard operating procedures (SOPs) for plant seed detection and pathegen identifiction. The SOPs will be useful for authorities of plant inspection and quarantine to detect the pathogenic bacteria of imported plant seeds to reduce and limit the introduction of plant pathogenic bacteria of Xanthomonas. 4. Development of detection system for important plant viruses of solanaceous seeds Tobacco rattle virus (TRV) and Tomato ringspot virus (ToRSV), which are important quarantine viruses, can infect vegetable seeds of the family Solanaceae. To prevent TRV and ToRSV being introduced into Taiwan through imported seeds, this project is going to develop the specific primers and antisera of TRV and ToRSV and thus establish a detection system for important seedborne viruses. The following works will be executed this year. (1) Collect長喙殼菌小蠹蟲木材檢疫選擇性培養基鑑別性培養基分子檢測法種子傳播病害種媒病毒煙草脆葉病毒番茄輪點病毒反轉錄聚合&#37238連鎖反應種媒病害土傳病害蛀果性昆蟲蛀仔性昆蟲Ophiostomabark beetlewood quarantineselective mediumdifferential mediummolecular detection methodseed-borne diseaseseed-borne virusTobacco rattle virusTomato ringspot virusRT-PCRseed-borne diseasesoil-borne diseasefruit borerse