Wang, X.X.WangYuan, T.T.YuanGuo, Z.Z.GuoHan, H.H.HanLei, Z.Z.LeiShimizu, K.K.ShimizuZhang, Z.Z.ZhangDUU-JONG LEE2021-02-042021-02-042020Wang, X.;Yuan, T.;Guo, Z.;Han, H.;Lei, Z.;Shimizu, K.;Zhang, Z.;Lee, D.-J.https://www.scopus.com/inward/record.url?eid=2-s2.0-85076029723&partnerID=40&md5=0faf6cd6dc9fa9654a0a9e77ca05cd68https://scholars.lib.ntu.edu.tw/handle/123456789/547649In this study, CH4 production from anaerobic digestion (AD) of refractory cellulose was investigated at a high loading of 3.5 (VScellulose/VSinoculum) under nanobubble water (NBW) addition. A longer proton spin-spin relaxation time (2611–2906 ms) of NBW during 35 days’ storage reflected its high mobility and diffusion of water molecules. Higher volatile fatty acids were yielded at the hydrolysis-acidification stage under NBW addition. Methanogenesis tests showed that Air-NBW and CO2-NBW supplementation accelerated the utilization of crystalline cellulose, achieving methane yields of 264 and 246 mL CH4/g-VSreduced, increasing by 18% and 10% compared to deionized water addition (the control), respectively. In addition, under NBW addition the cellulose crystallinity reduction was enhanced by 14–20% with microbial community being enriched with hydrolytic and methanogenic bacteria. Results from this work suggest that NBW environment with no chemical addition and relatively low energy consumption is advantageous for enhanced AD process of cellulosic biomass. © 2019 Elsevier LtdAnaerobic digestion; Cellulose crystallinity; Methane production; Nanobubble water; Proton spin-spin relaxation time[SDGs]SDG6[SDGs]SDG7[SDGs]SDG12Acidification; Cellulose; Crystallinity; Deionized water; Energy utilization; Hydrolysis; Methane; Molecules; Relaxation time; Volatile fatty acids; Cellulose crystallinity; Hydrolysis acidification; Hydrolysis and acidifications; Low energy consumption; Methane production; Microbial communities; Nano-bubble; Proton spins; Anaerobic digestion; biogas; cellulose; deionized water; methane; nanobubble; volatile fatty acid; water; methane; water; acidification; anaerobic digestion; cellulose; crystallinity; hydrolysis; methane; methanogenic bacterium; microbial activity; microbial community; acidification; anaerobic digestion; Article; biodegradability; controlled study; decomposition; diffusion; hydrolysis; kinetics; Methanobacterium; methanogenesis; methanogenic bacterium; microbial community; nonhuman; pH; priority journal; proton nuclear magnetic resonance; reduction (chemistry); species richness; zeta potential; anaerobic growth; bioreactor; dietary supplement; hydrolysis; Acidification; Cellulose; Crystallinity; Hydrolysis; Methane; Molecules; Anaerobiosis; Bioreactors; Cellulose; Dietary Supplements; Hydrogen-Ion Concentration; Hydrolysis; Methane; Waterjournal article10.1016/j.biortech.2019.122499318351462-s2.0-85076029723WOS:000504829800058