Lin, Wen-ChuanWen-ChuanLinLiao, Yuan-ChangYuan-ChangLiaoLiau, Ming-ChengMing-ChengLiauSheen, Lee-YanLee-YanSheen2010-07-262018-06-292010-07-262018-06-292003http://ntur.lib.ntu.edu.tw//handle/246246/190075This study investigated the effects of various concentrations and incubation time intervals of CDA-II (cell differentiation agent: a preparation of human urine) on cell viability, lipid peroxidation and glutathione and its related enzyme activities in rat hepatocytes. Based on the results of lactate dehydrogenase leakage and microscopic examination, treatment with CDA-II significantly elevated the viability of hepatocytes. The level of lipid peroxidation of cells treated with 2.5 or 5 mg/ml CDA-II was lower than the control after 4, 8 and 24 hours of incubation. Intracellular glutathione (GSH) content of cells treated with 0.25-5 mg/ml CDA-II was significantly higher than controls after 8 and 24 hours of incubation. These phenomena are beneficial to the antioxidant capabilities of hepatocytes. Furthermore, treatment of hepatocytes with CDA-II has no effect on the activities of GSH peroxidase, GSH reductase and GSH S-transferase. In conclusion, adequate concentration of CDA-II could enhance the viability and antioxidative capability of hepatocytes. ? 2003 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine.458766 bytesapplication/pdfen-USCDA-II; Glutathione; Hepatocytes; Human Urine; Rat[SDGs]SDG3cell differentiation agent II; glutathione; glutathione peroxidase; glutathione reductase; glutathione transferase; lactate dehydrogenase; peptide; phenylacetic acid; unclassified drug; animal cell; antioxidant activity; article; cell differentiation; cell viability; controlled study; drug effect; enzyme activity; incubation time; lipid peroxidation; liver cell; nonhuman; rat; Animals; Cell Differentiation; Cell Survival; Cells, Cultured; Dose-Response Relationship, Drug; Free Radical Scavengers; Glutathione; Hepatocytes; Humans; Lipid Peroxidation; Liver; Male; Peptides; Phenylacetates; Rats; Rats, Sprague-Dawley; Time Factors; Urinejournal articlehttp://ntur.lib.ntu.edu.tw/bitstream/246246/190075/1/23.pdf10.1142/S0192415X0300103Xhttp://ntur.lib.ntu.edu.tw/bitstream/246246/190075/1/23.pdf