Hsieh, Han-YunHan-YunHsiehChang, RayRayChangChuang, Er-YuanEr-YuanChuangFan, Yu-JuiYu-JuiFanWei, Pei-KuenPei-KuenWeiHORN-JIUNN SHEEN2024-07-032024-07-032023-01-019784886864352https://www.scopus.com/record/display.uri?eid=2-s2.0-85193479304&origin=resultslisthttps://scholars.lib.ntu.edu.tw/handle/123456789/719596Recent studies reveal that the severity of COVID-19 may be correlated with the presence of HHV-4. Although numerous devices can detect HHV-4 or SARS-CoV-2, to our knowledge, no device has been used to detect both simultaneously.Herein, we design a device that can perform a duplex polymerase chain reaction (PCR) in a microfluidic channel and detect amplicons through a surface plasmon resonance (SPR) sensor. This device can reduce the required PCR time by 55% compared to traditional instruments while yielding a similar amount of amplicon. Moreover, the device can detect desired genes by extracting cells infected with HHV-4/SARS-CoV-2.falseduplex PCRhuman herpesvirus 4label-freenanoslit-based plasmonicspolymerase chain reactionSARS-CoV-2[SDGs]SDG3conference paper2-s2.0-85193479304