2010-08-012024-05-16https://scholars.lib.ntu.edu.tw/handle/123456789/666730摘要：天然物自古即為重要的醫藥原料來源，時至今日大約有超過50%的藥品是由天然物衍生合成而來，因此，自天然物中尋找具有預防或抑制發炎反應的化合物為目前天然物界相當重視的研究主題。台灣杉（（Taiwania cryptomerioides Hayata）屬中生代與新生代第三紀孑遺植物，為世界稀有之珍貴樹種，木材性質良好，具有極佳的耐腐朽性及抗蟻性，為台灣原產重要經濟造林樹種。有關台灣杉成分分離及鑑定的研究工作已進行了數十多年，從台灣杉的木材、樹皮、根、針葉等各部位中分離鑑定出上百種成分，包括&#33820;類（Terpenoids）、木酚素（Lignans）、黃酮類（Flavonoids）及其他成分等。由先前的研究結果得知台灣杉具有一些獨特的二次代謝物，且台灣杉心材中具有極為豐富與多樣之木酚素與&#33820;類，為心材中主要的二次代謝物，過去十多年來，計畫申請人致力於探討臺灣杉抽出成分之生物活性（Bioacitvity），並評估其於保健醫療利用之潛力。本計畫將依生物活性為導向的分離策&#63862;（Bioactivity guided isolation strategy ） 與代謝體學（Metabolomics）的研究方法，自台灣杉中尋找具有抗發炎（Anti-inflammation）活性之木酚素與&#33820;類化合物，並進行一系列的體外試驗（In vitro）以及動物試驗等，期能釐清其抗發炎機制。<br> Abstract: Natural products have served as an important source of drugs since ancient times, and now more than 50% pharmaceuticals used today are derived from natural products. Prevention and treatment of inflammatory conditions is one of the important applications for natural products. Taiwania (Taiwania cryptomerioides Hayata) named after its native island of Taiwan. It is one of the relict plants from the Tertiary period of the Cenozoic era. As regard to the wood utilization of Taiwania, it is one of the most economically important tree species grown in Taiwan. By now, more than one hundred compounds, including terpenoids, lignans, isoflavones, and other compounds have been isolated from this species during the past seventy years. Among them terpenoids and lignans are the typical and dominant secondary metabolites in the heartwood of Taiwania. During the past decade, we have characterized the potent bioactivities of specific constituents from Taiwania, and evaluated the potential usages of the phytochemicals isolated from Taiwania for pharmacological applications. In this proposed project, the anti-inflammatory mechanisms of terpenoids and lignans, which are the major constituents existed in Taiwania will be investigated. The bioactivity guided isolation protocol and metabolomic strategy will be performed to find out the active anti-inflammatory compounds in Taiwania. Following by a series of in vitro and animal tests, we hope the action mechanisms of metabolites from Taiwania would be elucidated.台灣杉&#33820類木酚素抗發炎機制代謝體Taiwania cryptomerioidesterpenoidslignansanti-inflammationmechanismmetabolomics