Brown Seaweed Fucoidan Inhibits Cancer Progression by Dual Regulation of mir-29c/ADAM12 and miR-17-5p/PTEN Axes in Human Breast Cancer Cells

Wu S.-Y.Yan M.-D.Wu A.T.H.Yuan K.S.-P.SHING-HWA LIU2019-11-122019-11-1220161837-9664https://www.scopus.com/inward/record.uri?eid=2-s2.0-85006963777&doi=10.7150%2fjca.15703&partnerID=40&md5=c66e7e6528097e139db347beed90d09bhttps://scholars.lib.ntu.edu.tw/handle/123456789/431175In this study, we observed that brown seaweed fucoidan inhibited human breast cancer progression by upregulating microRNA (miR)-29c and downregulating miR-17-5p, thereby suppressing their target genes, a disintegrin and metalloproteinase 12 (ADAM12) and phosphatase and tensin homolog (PTEN), respectively. Moreover, fucoidan reduced the luciferase activity of 3'-untranslated region reporter; treatment of cells with the miR-29c mimic or miR-17-5p inhibitor also produced similar results. These effects of fucoidan inhibited the epithelial-mesenchymal transition in breast cancer cells, as evidenced by an increase in E-cadherin and a drop in N-cadherin, and inhibited breast cancer cell survival, as evidenced by the activation of the phosphoinositide 3-kinase/Akt pathway. Taken together, these findings demonstrate that fucoidan inhibits breast cancer progression by regulating the miR-29c/ADAM12 and miR-17-5p/PTEN axes. Fucoidan is a potential chemopreventive/chemotherapeutic agent for breast cancer. ? Ivyspring International Publisher.EnglishADAM12; Breast cancer cells; Fucoidan; MiR-17-5p; MiR-29c; PTEN[SDGs]SDG3ADAM protein; adam12 protein; antineoplastic agent; fucoidin; luciferase; microRNA; microRNA 17 5p; microRNA 29c; nerve cell adhesion molecule; phosphatidylinositol 3 kinase; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase; unclassified drug; uvomorulin; 3' untranslated region; antineoplastic activity; Article; breast cancer; cancer inhibition; cell survival; chemoprophylaxis; controlled study; down regulation; drug inhibition; enzyme activation; enzyme activity; epithelial mesenchymal transition; gene repression; gene targeting; human; human cell; MCF 7 cell line; MDA MB 231 cell line; reporter gene; seaweed; upregulationBrown Seaweed Fucoidan Inhibits Cancer Progression by Dual Regulation of mir-29c/ADAM12 and miR-17-5p/PTEN Axes in Human Breast Cancer Cellsjournal article10.7150/jca.157032-s2.0-85006963777