2012-04-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/682859摘要：囓齒類動物作為疾病治療研究的動物模式已經行之有年，因為其具有繁殖周期較短與低成本的優點，但是這些動物有許多生理反應與人類不盡相同，而且在藥物試驗上，它無法正確反應出可能的副作用，例如：嘔吐或藥效反應時程，所以由囓齒類動物取得的實驗數據不能完全代表人類的生理意義，也因此有些較大型動物模式 (如：豬與狗)更適合作為生醫研究與醫藥檢測之技術平台。因為豬的許多解剖和生理特徵與人類相似，尤其是心血管、代謝、骨骼與消化道都是如此，所以常被用於生醫研究。而且因其嗅覺敏感，在藥物試驗可以有效感受到藥物的異味而反應出其喜好程度，它也會因藥物副作用而產生嘔吐等行為讓科學家容易觀察，也使藥物的測試結果更接近人類的反應。 豬的品系很多，但都體型過大、不易操作、耗費人力與飼養空間、而且所需花費的飼料與藥物成本都相對較高，所以科學家一直在尋找替代方案。本校育種得到的李宋豬具有體型小、飼料成本低、飼養空間需求小，而且仍保由上述解剖生理與人相近的優點，所以目前為國內生醫研究模式的首選。李宋豬為本校自行雜交選拔育種超過30代之迷你豬品系，現階段李宋豬僅以保種為前提進行繁殖，尚未大量生產，所以如何有效率的生產品質一致、遺傳特徵相同的實驗動物，供相關生醫研究與醫藥篩檢平台，需要動科、獸醫、藥學與醫學等專家協力開發。因此希望透過本計畫的整合，建立李宋豬或蘭嶼豬在生醫研究與醫藥檢測之技術平台，發揮本校研發成果的實際應用與產業推廣價值，期能在未來與藥廠合作，讓本校開發出的品種與技術平台，可以造福更多的人群。我們設計5個研究從下列幾個方向著手：(1) 建立有效的生產最低病原豬系統以供應生醫研究所需李宋豬，並維持良好的品質控制；(2)以李宋豬為動物模式，釐清幾個重要人類疾病（例如：一型糖尿病、肥胖、脂性肝炎）的可能發生機制與治療模式；(3)確認機能性食品、益生菌或中草藥改善這些疾病的可能性及作用機制，希望在未來三年內將臺大的李宋迷你豬成為可以供生醫研究的良好動物模式，也讓本校的遺傳資源發揮更大的社會貢獻。 <br> Abstract: The application of rodent models in biomedical studies has been widely used for many years due to their low cost and short rodent proliferation time. However, rodent physiological characteristics are not similar to the human and cannot reflect the human side effects during pharmaceutical testing. Therefore, researchers try to find better representative animal models, such as swine and dogs for disease study. Swine have been applied in biomedical researches owing to their great similarity to humans in anatomy and physiology, including cardiovascular, metabolic, bone, and digestive systems. In addition, the sensitive olfaction and vomit reflect of swine enhances researchers easy observation of effects during pharmaceutical testing. The disadvantages of swine models in biomedical research are large size, difficult handling excessive consumption of power and space and high cost of feeds and drugs. Therefore, researchers try to find alternative models. Lanyu and Lee-Sung pigs are minipigs with smaller size, lower feeding costs and less space demands, but with maintenance of the great similarity to humans. All of these advantages make them the major animal model for biomedical research in Taiwan. Lee-Sung minipigs, developed by the experts of NTU, have been generated over 30 generations, but the quantity produced is small with proliferation for only maintenance. How to produce a large quantity of Lee-Sung minipigs and still maintain high genetic quality for biomedical study takes efforts from experts in animal technology, veterinary medicine, and pharmacy. Through the integration of this project, we will establish the platform of the Lee-Sung minipig model for disease study, increase the biomedical applications, and cooperate with pharmaceutical factories to develop the new drugs. Here, we propose 5 subprojects to establish the Lee-Sung Miniature pig to become a biomedical research model through (1) establishing an efficient production protocol and quality control of Lee-Sung pigs for biomedical study; (2) elucidating the exact mechanisms of human diseases (Type 1 diabetes, obesity and fatty liver) by using the Lee-Sung minipig model; (3) confirming the possibility of using herbs, probiotics, or functional foods to improve diseases.李宋豬一型糖尿病肥胖脂性肝炎Lee-Sung PigType I diabetesobesityfatty liver,