Chien, Shih-ChangShih-ChangChienChen, Miaw-LingMiaw-LingChenKuo, Hsiou-TingHsiou-TingKuoTsai, Yao-ChingYao-ChingTsaiLin, Bi-FongBi-FongLinKuo, Yueh-HsiungYueh-HsiungKuo2009-07-232018-07-062009-07-232018-07-06200800218561http://ntur.lib.ntu.edu.tw//handle/246246/162595http://ntur.lib.ntu.edu.tw/bitstream/246246/162595/1/29.pdfhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-51649095163&doi=10.1021%2fjf801171x&partnerID=40&md5=245b5751410e33d4c0b83b1d4c80e653Six new compounds, trans-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl] pyrrolidine-2,5-dione (1), trans- 1-hydroxy-3-(4-hydroxyphenyl)-4- isobutylpyrrolidine-2,5-dione(2), cis-3-(4-hydroxyphenyl)-4- isobutyldihydrofuran-2,5-dione (3), 3-(4-hydroxyphenyl)-4-isobutyl-1H-pyrrole-2, 5-dione (4), 3-(4-hydroxyphenyl)-4-isobutylfuran-2,5-dione (5), and dimethyl 2-(4-hydroxyphenyl)-3-isobutylmaleate (6), together with one known compound, 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]furan-2,5-dione (7), were isolated from the fruiting bodies of Antrodia camphorata. The structures of the compounds were elucidated by analysis of their spectroscopic data. To investigate the immunomodulatory potential of the compounds, RAW264.7 macrophage cells were treated with the compounds. Compound 1 significantly increased spontaneous TNF-α secretion from unstimulated RAW264.7 cells but suppressed IL-6 production [50% inhibition concentration value (IC50) = 10 μg/mL] in LPS-stimulated cells. Compounds 3, 4, and 6 also suppressed IL-6 production with IC50 values of 17, 18, and 25 μg/mL, respectively, suggesting that these four compounds may have an anti-inflammatory effect on macrophage-mediated responses. Of the six compounds, compound 1 was the most effective, exerting both immunostimulatory and anti-inflammatory effects. © 2008 American Chemical Society.application/pdf206978 bytesapplication/pdfen-USAntrodia camphorata; IL-6; Macrophage; Maleic acid derivative; Succinic acid derivative; TNF- αantiinflammatory agent; interleukin 6; maleic acid derivative; succinic acid derivative; tumor necrosis factor alpha; animal; Aphyllophorales; article; biosynthesis; cell line; chemistry; drug effect; fruiting body; immunology; macrophage; mouse; nuclear magnetic resonance spectroscopy; secretion; Animals; Anti-Inflammatory Agents; Cell Line; Fruiting Bodies, Fungal; Interleukin-6; Macrophages; Magnetic Resonance Spectroscopy; Maleates; Mice; Polyporales; Succinates; Tumor Necrosis Factor-alpha; Taiwanofungus camphoratus10.1021/jf801171x186428452-s2.0-51649095163WOS:000258633700034http://ntur.lib.ntu.edu.tw/bitstream/246246/162595/1/29.pdf