Lu M.-C.CHIA-LI YUChen H.-C.Yu H.-C.Huang H.-B.Lai N.-S.2021-01-272021-01-2720151462-0332https://www.scopus.com/inward/record.uri?eid=2-s2.0-84925581052&doi=10.1093%2frheumatology%2fkeu322&partnerID=40&md5=6ee74693c4c2518fa1afd9d174afbd40https://scholars.lib.ntu.edu.tw/handle/123456789/542170OBJECTIVE: The aim of this study was to investigate the pathogenic role of calcium (Ca(2+)) influx-regulated microRNAs (miRNAs) in T cells from patients with SLE.METHODS: Expression profiles of 270 human miRNAs in Jurkat cells co-cultured with or without ionomycin were analysed by real-time PCR. Differential expression of miRNAs in T cell samples from 28 patients with SLE (SLE T cells) and 20 healthy controls were investigated using western blot analysis of proteins expressed by respective miRNA target transcripts. Transfection studies were conducted to investigate miRNA-specific biological functions.RESULTS: Initial analysis revealed differential expression of nine miRNAs in Jurkat cells after co-culture with ionomycin. Of these, miR-524-5p and miR-449b were overexpressed in SLE T cells. Levels of expressed miR-524-5p showed a significant direct correlation with the SLEDAI. Transfection of Jurkat cells with miR-524-5p mimic suppressed Jagged-1 and Hes-1 protein expression. Likewise, expression of both Jagged-1 and Hes-1 proteins were diminished in SLE T cells. Upon activation of Jurkat cells transfected with miR-524-5p mimic, production of IFN-γ increased but the apoptotic rate was unaffected.CONCLUSION: In SLE T cells, miR-524-5p and miR-449b (both regulated by Ca(2+) influx) were overexpressed. Moreover, increased miR-524-5p expression, as shown by patients with SLE, directly paralleled disease activity (SLEDAI). Transfection of miR-524-5p also enhanced IFN-γ production in activated Jurkat cells. ? The Author 2014. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For Permissions, please email: journals.permissions@oup.com.Ca2+ influx; miR-449b; miR-524-5p; Notch; systemic lupus erythematosus; T cells[SDGs]SDG3basic helix loop helix transcription factor; calcium; calcium binding protein; gamma interferon; HES1 protein, human; homeodomain protein; membrane protein; microRNA; Serrate proteins; signal peptide; apoptosis; human; jurkat cell line; Lupus Erythematosus, Systemic; metabolism; physiology; secretion (process); T lymphocyte; Apoptosis; Basic Helix-Loop-Helix Transcription Factors; Calcium; Calcium-Binding Proteins; Homeodomain Proteins; Humans; Intercellular Signaling Peptides and Proteins; Interferon-gamma; Jurkat Cells; Lupus Erythematosus, Systemic; Membrane Proteins; MicroRNAs; T-Lymphocytesjournal article10.1093/rheumatology/keu322251729352-s2.0-84925581052