2005-08-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/659638摘要：由於醫藥科技的發達，大部分早期造成人類高死亡率的疾病已有許多藥物控制。然而現今許多新的疾病出現或者是已知疾病的變種，使得人類面臨新的威脅。面對捲土重來的已知疾病，開發快速檢測方式與有效藥物篩選，是所有生物製藥技術的共同目標所在，期望對於人類疾病進行有效的控制與治療，當今蓬勃發展之生物晶片即是一極具潛力的檢測方式。以結核病為例，此一疾病發病率逐漸攀升，貧困地區尤其受到嚴重威脅，每年死亡人數甚至超過愛滋病、瘧疾、腹瀉及熱帶病死亡人數之和。目前肺結核之診斷除了一般胸部X光檢查，主要是靠痰液之檢查。然而痰液檢查之敏感性不高且相當耗時，要蒐集多次痰液檢查且需經一個多月才能獲得完整之報告，因此經常延誤治療的時機。在卡介苗接種的成效一再被質疑之下，開發新的檢測方法以因應結核菌之大量篩檢，和快速且有效治療肺結核的藥物之篩選 是一重要的課題。 生醫晶片的相關檢測技術中，有以下發展的趨勢，即(1)為非接觸式(non-contact)，檢測不會影響被測體；(2)具高靈敏度(high sensitivity)、大頻寬(wide bandwidth)及小量測體積(small probe volume)等。同時<br> Abstract: Human beings have been threatened by tuberculosis for a long time. However, as the mortality rising gradually, especially those from depressed area, this problem is getting more seriously as time goes by. Death count that can be attributed to tuberculosis every year is more than the summation of those for AIDS, malaria, diarrhea and tropical diseases. In addition to x-ray photograph and test of skin tubercle bacillus, sputum test is currently the major way to diagnose tuberculosis. This method includes smear dyeing of mycobacteria and germiculture. However, sputum test suffers from low sensitivity and long detection time. Besides, repeatedly test, it usually takes more than a month to get a complete report. The opportune moment is wasted. While more the more people suspect the effect of BCG vaccine, it is necessary to develop new methods to handle large number of tubercle bacillus detection. The vigorously progressing biochip is a promising technique. The trends of biochip-related technique are as follows. (1) Use non-contact technique to avoid influence the sample. (2) To achieve high sensitivity, wide bandwidth and small probe volume. At the same time as the worldwide trend to reduce of the sample for biochip, opto-electric technique is the most suitable detection method. Out of all these opto-electronic techqniques, surface plasmon resonance, ellispsometry, and waveguide interferometry developed within the last year are some of the most promising techniques for wide spread use. Therefore, this sub-project will do the research on the development of the TBMorph (Tuberculosis Morph) in coordination with the main project to pursue the research on the “Opto-BioMorphin” that used in the epidemic detection and the drug development. In order to make TBMorph reliable and robust, cross calibration would be taken with instruments own by Bio-NEMS Group, like EP3 based on ellipsometry, GWC based on SPR, and Anilight Bio200 based on Waveguide Interferometry. By these procedures, the acquirements which are mass epidemic detection and the drug development could be achieved. On the other hand, the table of crosslink between these machines could also be established, and the ability of the drug development would be promoted. In addition, this team will also devote to reduce the size of this system and increase its accuracy. Ellipsometer and Surface Plasmon Resonance Detection will be integrated to build this Optomecharonic system to improve its performance, and therefore this instrument is called ESPR (Ellipsometer Surface Plasma Resonance). Besides, this sub-project will use ESPR to develop the drug of Tuberculosis so that a system that can be used both in the detection and drug development of Tuberculosis.光生化檢測儀生醫晶片橢偏儀表面電漿共振儀波導干涉儀Opto-BioMorphinOBMorphbiochipellipsometerSurface Plasmon ResonanceWaveguide Interferometry