HUNG-YUAN LIMAO-SHIN LINWei J.-N.CHI-SHENG HUNGFU-TIEN CHIANGLin C.-H.Hsu H.-C.Su C.-Y.Wu M.-Y.Smith D.J.Vainio J.MING-FONG CHENLEE-MING CHUANG2020-06-012020-06-0120090009-8981https://www.scopus.com/inward/record.uri?eid=2-s2.0-64849117833&doi=10.1016%2fj.cca.2009.01.027&partnerID=40&md5=250957ca4d9972362dcabccfe7a99641https://scholars.lib.ntu.edu.tw/handle/123456789/495544Background: We investigated if serum vascular adhesion protein-1 (SSAO/VAP-1) changed acutely following oral glucose loading and whether such changes are correlated with surrogate markers of atherosclerosis. Methods: A total of 115 non-diabetics subjects were enrolled for an oral glucose tolerance test (OGTT). Carotid intima-medial thickness (IMT) was measured by ultrasonography. Serum SSAO/VAP-1 was analyzed by time-resolved immunofluorometric assay. Serum thiobarbituric acid reactive substances (TBARS) and advanced glycated end products (AGEs) were measured by fluorometric assays. Results: Serum SSAO/VAP-1 increased significantly at 30?min after oral glucose loading and lasted to 2?h (p = 0.0005 and p < 0.0001, for 30?min and 2?h respectively). The area under curve of serum SSAO/VAP-1 during OGTT (AUC-VAP-1) correlated significantly with carotid IMT, independent of age, gender, low-density lipoprotein cholesterol, systolic blood pressure, hemoglobin A1c, serum TBARS, AGEs, and high-sensitivity C-reactive protein. Subjects with a positive AUC-VAP-1 had significantly higher serum TBARS and AGEs than subjects with a negative AUC-VAP-1 adjusted for age and gender. Conclusions: Serum SSAO/VAP-1 changed acutely following oral glucose loading in non-diabetic subjects. Change of serum SSAO/VAP-1 correlated independently to serum TBARS, AGEs, and carotid IMT. Our findings suggest that acute change of serum SSAO/VAP-1 is a novel marker for hyperglycemia-induced atherosclerosis. ? 2009 Elsevier B.V. All rights reserved.[SDGs]SDG3advanced glycation end product; C reactive protein; hemoglobin A1c; low density lipoprotein cholesterol; thiobarbituric acid reactive substance; vascular adhesion protein 1; adult; article; atherosclerosis; carotid artery; controlled study; female; human; immunofluorescence test; intima; major clinical study; male; oral glucose tolerance test; priority journal; systolic blood pressure; tunica media; ultrasound; Amine Oxidase (Copper-Containing); Atherosclerosis; Carotid Arteries; Cell Adhesion Molecules; Diabetes Mellitus; Fasting; Female; Glucose; Glycosylation End Products, Advanced; Humans; Male; Middle Aged; Tunica Intimajournal article10.1016/j.cca.2009.01.027193614612-s2.0-64849117833