2006-10-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/711955摘要：近年來因為基礎生物分子研究的蓬勃發展，相關生物分子應用科技上有愈來愈多的研究成果發表，其中利用生物體內之分子馬達來擷取生物能源亦引起廣泛的注意。因為體內環境(in vivo)與體外環境(in vitro)的不同，生物分子馬達的運作模式亦會有所不同，如何有效的於體外環境中運用生物分子馬達即成為重要並值得深入探討的課題之一。 本計畫將針對運輸蛋白(Kinesin)及微管(Microtubule)這一組生物分子馬達，建構其於奈微米流體管道中的運動模型並依據此一模型提出相關奈微米流體管道的設計準則。於細胞體內環境，微管為一固定之軌道而運輸蛋白可沿著微管進行運動，有別於體內環境的運作，應用於奈微米流體管道時，運輸蛋白為固定於元件表面而微管則於運輸蛋白上移動，本計畫將以對微管機械性質的瞭解為基礎、統計力學為工具，先以理論分析的方式來建構微管的移動模型，並針對微管於奈微米製程元件中移動時受到奈微米流體管道形狀的影響進行進一步的分析，與現有之實驗結果驗證模型之正確性。此一模型的建立不但可以提供生物分子馬達相關應用的奈微米流體管道之設計準則，更可以此一模型為基礎對於在不同的外在環境下，諸如電場或流<br> Abstract: Biomolecular-NEMS will harness the unparalleled performance and scale of biological molecules and advanced NEMS to create small, inexpensive, robust and efficient devices at the nano- to micrometer scale. Among the various biomolecular-NEMS applications, the energy harvesting achieved by biomolecular motor proteins received the major attention because of its compact size and high energy transfer efficiency. Utilizing nano/micro fabrication technology, the biomolecular hybrid devices can be implemented to extract the power from motor proteins. However, there is no quantitative analysis of the motor protein movement affected by device topography in micro-structural design point of view. To promote the effective driving mechanism from motor proteins in these devices, it is important to understand the motor protein moving behavior in nano/micro fabricated devices To establish the understanding of motor protein movements in vitro, this project will aim at the motor protein, kinesins, which is one of the major proteins used to actively transport substances in cells. The motor protein, kinesin, holds significant potential for nano-scale actuation applications because it is compact, efficient (~ 50%), and moves robustly in vitro. In vivo, it generates linear, stepwise motion along microtubules (a filamentous cytoskeletal polymer) toward their plus-end by using hand-over mechanism of its two motor domains. On the other hand, the microtubule is gliding on the kinesin coated surface as integrating them into nano/micro fabricated devices. Because of this fundamental operation difference between in vitro and in vivo, this research project will construct the microtubule moving model in vitro. Utilizing the understanding of microtubule mechanical characteristics, the microtubule movement model in vitro can be established by using statistical mechanical analysis. By accounting for the physical interaction between microtubules and fabricated channels, the developed model can be used to compare with the experimental results presented in previous works. Based on this work, we will deliver the basic design rule of constructing active transportation systems driven by motor proteins. In addition, the developed model can also be used in analyzing the microtubule movement in other applied external fields, such as the flow field or electrical field. As the consequence, the guide line of the engineered nano/micro devices driven by biomolecular motor proteins can be established.運輸蛋白微管生物分子馬達奈微米流體管道motor proteinkinesinmicrotubulenano/micro fluidic channel