2015-09-012024-05-16https://scholars.lib.ntu.edu.tw/handle/123456789/668354摘要：本計畫欲利用台灣多組研究團隊，與美國南加大鍾正明院士的研究團隊相互合作，目的目標是希望能透過細胞層次之分子機轉與神經迴路之運轉模式，來解析鳥類在視覺刺激下之擇偶決策；並且，更長遠來說，是希望在台灣生根，建立以轉殖基因鳥禽類為模型之研究平台。本計畫的工作項目包含以下幾點: (1) 學習並建立以鳥禽類動物實驗平台之認知研究模型，包括展示者 – 感應器 – 訊息處理中心之軸式模型，及學習基因導入與基因轉殖鳥禽類之發育與分子生物學技術，(2) 分析 「展示者」- 羽毛色素形成與排列模式之兩性異型分子機轉，(3) 分析 「感應器」- 視網膜感光細胞之排列分布，光譜分析，與刺激網膜電位圖分析 (ERG)，(4) 三層次解析 「訊息處理中心」- 神經迴路與腦區分析，包括神經電生理 (LFP 及 EEG) 腦區分析，c-Fos 免疫染色與影像學觀察定位，以及高解析度鈣影像神經活性定位定量分析。以上這四種目標將由台灣以及美國的團隊相互分工完成，並且將保持緊密的合作，將四個主要研究方向相互連結並且致力達到設立的目標，並將核心技術帶回台灣，期使台灣在以鳥禽類動物模型之研究領域中有顯著進步及突破。<br> Abstract: The main goal of this project is to understand the molecular, cellular, and neuro-cognitive basis of visually based mating choice using the avian model and, furthermore, to use this opportunity to set up a long term multi-disciplinary research platform in Taiwan. We propose to set up such a multi-disciplinary team to tackle this question with experts and resources from National Taiwan University and the University of Southern California including researchers in the fields of developmental biology, ophthalmology, neurobiology, and animal behavior. The investigations include four parts executed by the research groups from both sides, i.e. (1) learning and setting up the bird platform for cognition studies – including depicting the cognition pathway of the Displayer – Sensor – Processor axis, learning developmental biology and gene transduction for birds, and learning the technique of generating transgenic quail and establish the technical platform on the finch, (2) analyses of the displayer – the sexually dimorphic pigment patterns and feather forms, (3) analyses of the sensor – retina, with the analysis of photoreceptor distribution and electroretinogram (ERG), (4) analyses of the processor – brain, with a three-tiered approach: electrophysiology for crude mapping (LFP and EEG), immunohistochemistry with c-Fos for finer mapping, and calcium imaging for high resolution mapping. We expect the close collaboration among the research groups ensures the inter-connection of the four investigation directions that are helpful for achieving significant advance in the transgenic avian research platform and related research work.基因轉殖鳥禽視覺處理認知神經迴路transgenic avianvisual processcognitionneural circuits