Shih-Chung WeiTsung-Liang ChuangDa-Shin WangHui-Hsin LuFrank X. GuKung-Bin SungCHII-WANN LINKUNG-BIN SUNG2018-09-102018-09-102015-02http://scholars.lib.ntu.edu.tw/handle/123456789/394775A tip nanobiosensor for monitoring DNA replication was presented. The effects of excitation power and polarization on tip-enhanced fluorescence (TEF) were assessed with the tip immersed in fluorescein isothiocyanate solution first. The photon count rose on average fivefold with radially polarized illumination at 50 mW. We then used polymerase-functionalized tips for monitoring loop-mediated isothermal amplification on Hepatitis C virus cDNA. The amplicon-SYBR? Green I complex was detected and compared to real-time loop-mediated isothermal amplification. The signals of the reaction using 4 and 0.004 ng/μ l templates were detected 10 and 30 min earlier, respectively. The results showed the potential of TEF in developing a nanobiosensor for real-time DNA amplification. ? 2015 Society of Photo-Optical Instrumentation Engineers.Hepatitis C virus; loop-mediated isothermal amplification; radial polarized illumination; tip-enhanced fluorescence[SDGs]SDG3Fluorescence; Viruses; DNA amplification; DNA replications; Enhanced fluorescence; Fluorescein isothiocyanate; Hepatitis C virus; Loop mediated isothermal amplifications; Polarized illumination; Radially polarized; Isotherms; Hepatitis C virus; complementary DNA; fluorescein; fluorescent dye; virus RNA; chemistry; devices; fluorescence microscopy; genetics; Hepacivirus; nanotechnology; nucleic acid amplification; procedures; DNA, Complementary; Fluorescein; Fluorescent Dyes; Hepacivirus; Microscopy, Fluorescence; Nanotechnology; Nucleic Acid Amplification Techniques; RNA, Viraljournal article10.1117/1.JBO.20.2.0270052-s2.0-84923238308WOS:000350462900022