Hsu, Hung-ChuanHung-ChuanHsuVyas, SunilSunilVyasChu, Cheng HungCheng HungChuJUI-CHING WUTanaka, TakuoTakuoTanakaTsai, Din PingDin PingTsaiHSIEN-SHUN LIAOKUANG-YUH HUANGYUAN LUO2025-09-172025-09-172025-09-01https://www.scopus.com/record/display.uri?eid=2-s2.0-105014127811&origin=resultslisthttps://scholars.lib.ntu.edu.tw/handle/123456789/732151Light-sheet fluorescence microscopy (LSFM) is an essential imaging system for observing biological samples with high contrast, low phototoxicity, and rapid image acquisition. Traditional LSFM utilizes Gaussian illumination with a limited system field of view (FOV) due to the short Rayleigh range. As one kind of the propagation-invariant light field, the Airy beam can inherently extend the FOV and enhance the axial resolution for microscopy. However, implementing an Airy-LSFM requires a complex illumination system. Here, we demonstrate that the Airy light-sheet is conveniently integrated into LSFM using a polarization-independent, Airy metasurface to achieve compact illumination, large system FOV, and high image quality. To verify the performance of the metasurface-based Airy LSFM (meta-Airy LSFM), ex vivo imaging of fluorescently labeled Caenorhabditis elegans (C. elegans) embryos was conducted. The Richardson-Lucy deconvolution technique was applied to further improve the image quality. The experimental results show that the axial and lateral resolution of the meta-Airy LSFM is 2.3 and 0.69 μm, respectively, while the FOV is significantly improved tenfold, up to 200 μm. The tightly packed integration of the Airy light-sheet metasurface in LSFM enables wide-ranging biological applications and has great potential to be easily adapted across various LSFM modalities.[SDGs]SDG3journal article10.1063/5.0264429