國立臺灣大學分子與細胞生物學研究所高燕玉2006-07-252018-07-062006-07-252018-07-062005http://ntur.lib.ntu.edu.tw//handle/246246/5049核糖體RNA 基因包括18S-5.8S-26S rDNA（即45S rDNA）與5S rDNA 兩種，均為聚集型的重複性序列，此兩種基因的重複單位皆包含轉錄區及基因間間隔區(IGS)，其中18S 與5.8S 間及5.8S 與26S 間又被內部轉錄間隔區(ITS)隔開，分別為ITS1 和ITS2。ITS 和IGS 之序列及長度在物種間的差異很大，因此常用於親緣關係較近物種間的分析。本實驗以 Phalaenopsis aphrodite 之5S rDNA 的基因轉錄區設計一對專一性的引子，利用聚合酵素連鎖反應（PCR）擴增28 種蝴蝶蘭及1 種朵麗蘭植物之IGS 序列，做親緣關係樹的分析，可將28 種蝴蝶蘭分成7 群。此結果與過去利用ITS 序列分析的結果相比較，共有四群植物相吻合，同時IGS 序列分析的結果，與傳統依植物形態分類的結果較為一致。18S-5.8S-26S (45S) and 5S rDNAs are organized into clusters of tandem repeat with each repeat consisting of a transcribed region, and an intergenic spacer (IGS). In the transcribed region of 45S rDNA, on either side of 5.8S rDNA are found internal transcribed spacers (ITS), described as ITS1 and ITS2. The ITS and IGS have sequences diverged more than the transcribed region, and the presence of highly conserved sequences flanking each of ITS and IGS make them easy to amplify and useful for phylogenetic analyses of interspecies. In this study, the IGS sequences of 28 Phalaenopsis species and one Doritis species were amplified by the polymerase chain reaction (PCR) which used specific primers devised by 5S rDNA transcribed region of P. aphrodite. The neighbor-joining tree constructed from IGS consensus sequences divides the 28 Phalaenopsis species into seven groups. The species from four groups coincided with the results from the IGS and ITS sequences analyses. Additionally, species in IGS phylogenetic tree were in agreement with traditional taxonomic status.application/pdf163815 bytesapplication/pdfzh-TW國立臺灣大學分子與細胞生物學研究所蝴蝶蘭基因間間隔區親緣關係PhalaenopsisIGSphylogenyreporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/5049/1/922317B002021.pdf