2004-05-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/650796摘要：本計畫乃是台灣茄科基因體計畫之一，也是因應加入國際茄科基因體計畫所進行的研究，目的是要瞭解茄科植物不同物種間的歧異度與適應力。番茄因它的營養與預防人類攝護腺癌的功效而大受歡迎，其中茄紅素是主要的聚焦點。茄紅素是類胡蘿蔔素生合成的重要中間產物，它可以進一步分別轉變成luteins與neoxanthin。此合成途徑中主要的調控點被認為是在第一步驟，即由phytoene synthase(PSY)將2分子的geranylgeranyl diphosphate(GGDP)轉變成phytoene；然而光所誘導的茄紅素含量增加的調控機制，目前並不十分清楚。所以，本計畫的目標是瞭解茄紅素合成的調控機制，特別是受光影響的調控因子的功能。目前已知一些影響茄紅素含量的突變體，如番茄high pigment突變體hp-1與hp-2；兩者累積大量的花青素並且具有對光高度敏感的短下胚軸外表型；除此之外，亦含顯著量的類胡蘿蔔素，其中最主要是果實中的茄紅素與類黃體素（flavonoids）。另外Alba等人（2000）報導果實中的光敏素可促進茄紅素含量增加2.3倍，暗示光敏素調控的訊息傳遞參與此過程。故本計畫擬利用<br> Abstract: This project is one of Taiwan Solanaceae Genome Projects, which join the International Solanaceae Genome Project, aiming at the understanding of diversity and adaptation among the species in Solanaceae. Tomato is popular with the fact of its nutrition and medicinal value of protections of the prostate cancer in human. The lycopene level becomes the main focus for its popularity. Lycopene is an important intermediate during the biosynthesis of carotenoids, and it can be further converted into luteins and neoxanthin, respectively. The committed step in the pathway has been thought to be in the conversion of 2 molecules of geranylgeranyl diphosphate(GGDP) to phytoene by phytoene synthase(PSY). However, the regulation of lycopene levels by light is still not clear yet. So the objective of the project is to get the insight of how lycopene biosynthesis is regulated by the integration of the biosynthetic pathway and other regulatory factors, especially those affected by light.Several mutants affecting lycopene levels have been isolated. Tomato high pigment(hp) mutants, hp-1 and hp-2 accumulate high levels of anthocyanin and display a hypersensitive response to light showing a shorter hypocotyl phenotype. In addition, both mutants also contain significant amount of carotenoids, in which most of them are lycopene and flavonoids in their fruits. Alba et al.(2000) reported that fruit-localized phytochromes regulate lycopene accumulation by 2.3 times increase, suggesting that phytochrome-regulated signal transductions are involved in this process. So, the project using a DNA microarray approach is to carry out the functional genomic studies of regulatory factors affecting light-induced lycopene level in heat-tolerant tomatoes. Yearly work of the project is described as follows: The first year is to obtain those factors induced by red light. The second year is to identify regulatory factors influencing the lycopene level from those red light-regulated factors. The third year is to establish regulatory relationships among these regulatory factors. By the accomplishment of the project, it will definitely set up the further understanding of how lycopene levels are regulated at molecular level and then we can apply the information of regulatory mechanisms to the molecular engineering of lycopene contents, hoping to control the lycopene level and improve the economic profits of tomatoes.番茄茄紅素光敏素高色素含量的突變體tomato lycopenephytochromeshigh pigment mutants