2010-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/691711摘要：本世紀淡水將逐漸變得稀有。由於人口的增加與經濟發展世界上地表水的抽取速率在未來二十年預估將呈倍增。農業是淡水的主要消費者。世界上某些地區由於氣候暖化淡水的缺乏將更為嚴重。植物的基因体中的轉錄子不僅較其他真核生物有較高的重複突變率，同時在植物的各類基因中亦有更高的擴張速率。轉錄子由於可影響到許多逆境相關目標基因之表達己証實在改良植物逆境耐受力方面‧具有很大的用處。許多具有AP2 domain的轉錄子在乾旱逆境下可被活化。是否這些轉錄子均參與在乾旱的訊息傳導上呢？在本計劃內我們將大量篩選轉錄子過度表現的變異株並進行功能上的分析將可對它們是否參與乾旱逆境獲致一些答案。AtTORF-Ex種子庫收集了過度表達含有HA標記的轉錄子基因序列的變異株將用於耐旱功能之篩選以找出特定的轉錄子。找到具有耐旱能力的轉錄子將進行遺傳上與生理上的特性分析。我們除了要確定篩選到的轉錄子具有改善植物耐旱的功能之外，轉錄子在細胞內的分佈位置，&#32452;織專一性，蛋白質之活化區域與負調控區，與轉錄子下游所調控之基因亦將加以研究並用來解釋過度表達何以耐旱之機制。本研究方法在強調表達基因之功能性，因此可解釋其逆境耐受性之功能。<br> Abstract: Freshwater is a natural resource that becomes increasingly scarce in the 21st century. Because of increased population growth and economic development, global withdrawal rates of surface water are projected to at least double in the next two decades. Worldwide, agriculture is the primary consumer of freshwater. Water shortages may become even more acute in some areas as a result of global climate change. Transcription factors (TF) not only have higher duplication rates in plant genomes but also to a higher degree of expansion compared to other plant genes. TFs have proven quite useful in improving stress tolerance in transgenic plants, through influencing expression of a number of stress-related target genes. Many AP2 domain transcription factors show activation under dehydration condition. Do they all involve in dehydration signal pathway? In this project, large scale screening of overexpression AP2 domain TF mutant lines and functional analysis will answer part of the question. AtTORF-Ex library, a collection of transgenic lines that over-express HA-tagged AP2/ERF coding sequences will be used in various functional gene-mining screening to identify particular gene family members involved in stress responses. Selected transcription factors tolerant to dehydration will be characterized genetically and physiologically. We will analyze the stress tolerance of the transgenic plants to see whether improvement of drought stress tolerance can be obtained. In addition, subcellular localization of selected transcription factors, tissue specificity, activation domain and negative regulation domain, if exist, and the up-regulated and down-regulated genes will be examined by micrarray to explain the mechanism of dehydration capacity. This approach emphasizes the functionality of the expressed genes, and therefore the cloned genes are directly linked to their function in stress tolerance.阿拉伯芥AP2轉錄子乾旱誘導功能性基因体微陣Arabidopsis thalianaAP2 domain transcription factordehydration induciblefunctional genomicsmicroarray.