Lin HKUAN-CHEN CHENGLin J.-AHsieh L.-PChou C.-HWang Y.-YLai P.-SChu P.-CHsieh C.-W.2022-12-142022-12-14202220763921https://www.scopus.com/inward/record.uri?eid=2-s2.0-85127651313&doi=10.3390%2fantiox11040739&partnerID=40&md5=c524447acbc7b1571b34d1bee2729f9bhttps://scholars.lib.ntu.edu.tw/handle/123456789/626121Ultraviolet-A (UVA) exposure is a major cause of skin aging and can induce oxidative damage and accelerate skin wrinkling. Many natural polysaccharides exhibit a UV protective effect. In research on Pholiota nameko polysaccharides (PNPs), a natural macromolecular polysaccharide (4.4–333.487 kDa), studies have shown that PNPs can significantly decrease elastase activity to protect against UVA-induced aging in Hs68 human dermal fibroblasts. Cellular experiments in the present study indicated that PNPs can protect against UVA-induced oxidative damage in Hs68 cells by inhibiting the production of reactive oxygen species. Furthermore, PNPs significantly attenuated UVA-induced cell aging by decreasing the protein expression of matrix metalloproteinase 1, 3, and 9. Pretreatment of Hs68 cells with PNP-40, PNP-60, and PNP-80 before UVA irradiation increased protein expression of tissue inhibitor metalloproteinase 1 by 41%, 42%, and 56% relative to untreated cells. In conclusion, this study demonstrates that PNPs are a natural resource with potentially beneficial effects in protecting against UVA-induced skin aging. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.aging; human dermal fibroblasts; oxidative damage; Pholiota nameko polysaccharides; ultraviolet-A[SDGs]SDG3journal article10.3390/antiox110407392-s2.0-85127651313