2011-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/684938摘要：本計畫主要目的在於利用布朗運動感測技術，開發一個微型化生物感測元件的前期作業。其具有操作簡易、不需利用微機電製程、易整合於微流體元件中及高靈敏度檢測等優點。本研究將以實驗室現有布朗運動量測技術為基礎，對此感測技術進行最佳化設計，將其應用於蛋白質、病毒等生物分子之感測，企圖推廣以及給予該感測技術最好的應用定位。本計畫並將嘗試開發一個整合型晶片利用電場操控液珠方式，達到一個高效能的生物分子感測陣列晶片，最後整合該晶片於攝影功能元件，期待未來可整合於具有攝影功能的攜帶式電子產品，例如：具有照相功能的手機。本期研究之重點為，內容分述如下：將針對微流道中布朗運動的現象做一個完整的探討，包括布朗運動受壁面影響的效應等等，預期將實驗與理論預測進行比較，並以此實驗結果將過去開發的血清液體黏性、及C反應蛋白濃度感測技術最佳化。 <br> Abstract: The developments of bio-sensor chip on miniaturized devices have been substantially becoming important issues during the past few years. In one our previous project, a quantitative immunosensing technique based on the measurement of nanobeads’ Brownian motion was development by our group. An advantage of the technique is that it is micro-electro-mechanical-system (MEMS) fabrication free technique and shows highly compatible ability with any lab-on-a-chip device. We propose a new three-year project of this miniaturized bio-detection system based on Brownian measurement introduced as follows: The objectives of this proposed experimental study are firstly to set up a Brownian motion analysis system by using micro Particle-Tracking-Velocimetry (μ-PTV) technique to measure the nano-particles in the scales of 50 nm to 700 nm and to try to observe the flow field by Total Internal Reflection Fluorescence Microscopy (TIRFM) system. Moreover, the combined effects of high concentration and boundary on particles’ Brownian motion will be investigated by this new sensing technique. Secondly, the Brownian motion sensing mechanism will be conducted for the orchid’s virus detection and to be used to demonstrate whether this method can be used for wild bio-sample detection including biomarker protein and virus. The goal of this project is that a modified TIRFM-μPTV system will be set up as the measurement apparatus. The nano-particle Brownian motion behaviors will be investigated experimentally. Because the Brownian motion displacement will be changed by the particle size which will bind antibody-antigen onto the sphere surface. It will be use to develop a new bio-sensor. Further, the process of bio-molecule binding will be tried with chemical treatments.布朗運動微粒子影像追蹤測速儀全反射螢光顯微鏡微型化生物分子感測裝置Brownian motionμ-PTVTIRFMminiaturized bio-molecule sensor