內科TSENG, CHIN-HSIAOCHIN-HSIAOTSENG2008-12-292018-07-112008-12-292018-07-112001http://ntur.lib.ntu.edu.tw//handle/246246/95168Objective: To develop a real-time PCR technique for detection of the insertion/deletion (YD) polymorphism of angiotensin-converting enzyme (ACE ) gene.Design and methods: Three primers were designed for performing real -time PCR in the presence of SYBR Green I as flurochrome followed by melting curve analysis. Forty human genomic DNA that have been genotyped by two-rounds of conventional PCR were used for evaluation of this technique.Results: Melting curve analysis indicated the melting peak at 73 .9degreesC and 76.2 degreesC corresponding to the presence of I and D alleles, respectively. Comparable genotyping results were obtained by both conventional and real-time PCR. Besides, the mistyping of ID allele individuals by the first run of conventional PCR were accurately genotyped by single-tube real time PCR. Conclusions: The real-time PCR method presented in this study provides a rapid and sensitive way for genotyping of ACE gene that may be suitable for large-scale clinical and epidemiologic study. (C) 2002 The Canadian Society of Clinical Chemists. All rights reserved.en-USangiotensin-converting enzymereal-time PCRinsertion/ deletion polymorphismDELETION POLYMORPHISMHEART-DISEASEACE GENE[SDGs]SDG3journal article