2015-05-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/649169摘要：兒童的呼吸道感染性疾病一直是兒童健康的最大議題，因此建立一個全國性跨領域的臨床試驗聯盟，以及完備的呼吸道病毒資料庫乃刻不容緩。因此，由台灣小兒感染症聯盟（TPIDA）建立之台灣小兒感染症臨床試驗合作聯盟，將可提供為本土重要呼吸道病毒感染疾病之合作平台。台灣小兒感染症聯盟建立於 2009 年，由台大小兒感染科黃立民教授主持，乃國內八家醫學中心的小兒感染科共同成立，過去兩年來已有成功執行全國性的兒童肺炎研究之經驗。以TPIDA 為基礎，我們將可以成功收集病人的呼吸道病毒、病人的臨床檢體及其臨床資料，成立呼吸道病毒資料庫，橫向的整合作為將來的臨床研究、基礎研究、和產學合作的平台。台灣小兒感染症臨床試驗合作聯盟由臨床出發與學界及實驗室整合，可以更方便提供疫苗，抗病毒藥物治療，與新的檢驗方法之臨床試驗。我們的呼吸道病毒資料庫之特色，除了傳統病毒培養以及聚合酵素鏈鎖反應之外，將納入人類呼吸道上皮細胞培養的技術（HAE），增加敏感性與完備性。對於目前未知的病毒，也可能藉由此技術而有新的發現。<br> Abstract: Children bear the heaviest burden of infectious illness. An island-wide or even international multi-institution collaboration is especially needed to accomplish any clinical trial in the field of pediatric infectious diseases. This is the grounds that we established the first Taiwan Pediatric Infectious Diseases Alliance (TPIDA) in 2009.TPIDA, led by Professor Li-Min Huang of National Taiwan University Hospital, is a collaborative consortium of pediatric institutes in eight major medical centers around the island: National Taiwan University Hospital, National Cheng Kung University Hospital, Mackay Memorial Hospital (Taipei), Chang Gung Memorial Hospitals (Linkou Branch and Kaohsiung branch), Hualien Tzu Chi Hospital, China Medical University Hospital, and Veterans General Hospital (Taichung). Since 2010, we together have already accomplished many joint researches in regard to pediatric lower respiratory tract diseases, and more studies about vaccine efficacy and effectiveness are on the way.Now in TPIDA, we have about 20 core members, including infectious diseases specialists, virologists, molecular biologists, and more than 30 technicians and assistants working together. It is believed that in 3 years, we will be able to establish a platform helpful for conducting clinical research activities in Taiwan. The database management platform is already established, and is proved working after a 4-year long collaborative study of pediatric pneumonia.The second aim is to build up the only respiratory tract virus bank in Taiwan. There are mainly two ways to detect viral pathogens from clinical respiratory tract samples: viral culture in animal/human cell lines, or PCR. However, there are two major disadvantages. First, not all viruses are culturable in cell lines. Besides, one can only find out known viruses through these two methods. Furthermore, immortalized tissue culture cells are adapted to growth in laboratory conditions and as such, display altered gene expression patterns, which may not be optimal for replication of fastidious viruses. Except for using conventional virus culture and PCR method, we will integrate the (HAE) system into the collection of respiratory virus bank. This unique culture system, although labor-intensive, is believed to be the key of finding novel respiratory pathogen that has not yet discovered. The utilization of the culture system will expand to become a part of our respiratory tract virus bank. All clinical samples in which no known virus could be detected will be cultured in HAE system and stored. Any future translation research activities can utilize the clinical samples in the respiratory tract virus bank.Taiwan Clinical Trial Consortium for Pediatric Infectious Diseases steering meeting is held trimonthly, where updated data on various aspects are generated and discussed. Our aim is to expand this center from Taiwan Pediatric Infectious Diseases Alliance to an international collaboration. And we certainly will.兒童呼吸道系統病毒臨床試驗人類呼吸道上皮細胞培養PediatricsRespiratory systemVirusesClinical Trial ConsortiumPrimary Cell Culture