2005-01-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/654536摘要：植物生長發育調控及環境逆境為影響產量及品質之重要因素。根據統計，鹽害、乾旱及溫度等非生物性環境逆境平均造成重要作物之生產降低 50%以上。此整合計畫將以T-DNA tagged 水稻突變庫基礎篩選出與生長發育（例：穎花發育）及環境逆境（包含：高鹽、乾旱、溫度逆境）相關之基因。此整合計畫將採分工系統進行，本子計畫在此系統中主要負責陸續釣取穎花發育、高鹽、乾旱及溫度逆境相關突變系之T-DNA tagged gene 之cDNA或啟動子，並進行序列及表現分析。進而，將此些分析結果及DNA提供給其他子計畫進行進一步研究及應用。此外，亦計畫將此研究所篩選出之逆境、發育相關基因集中製成cDNA array，此cDNA array將應用於探討其他不同環境逆境，例如重金屬、氧化逆境、生物逆境等相互間之關係，並可將所有研究資訊擴展應用到其他單子葉植物，例如玉米、小麥等之研究。 此外，本子計畫亦將針對鹽害逆境相關基因及啟動子進行功能及調控研究。全球有越來越多的地區面臨無足夠淡水以供作物灌溉之困境。全球已有20%的可耕地具有鹽害問題，如果以灌溉土壤而言，則50%具有鹽害。而台灣為一海島，因長期抽取地下水而<br> Abstract: Coordination of endogenous developmental factors and environmental effects is the major factor to determine the crop yield. Some abiotic stresses are responsible for reducing the crop yield more than 50%. The goal of this consortium project is to isolate and characterize the rice development and abiotic stress-related genes (e.g. salt, drought and cold stress) for studying their physiological functions and applying them to improve the production of rice. In network system of this consortium project, my laboratory is in charge of using molecular approaches to isolate and characterize the target genes or promoters of the T-DNA inserted mutants related development and stress tolerance selected by other laboratories (Dr. Li-Fei Liu, Wen-Lii Huang) in this team. Besides, these characterized Cdna will be collected and made to be the stress-cDNA array for providing to stresses cross-talk study. It is expected my lab as a hub to identify genes for necessary of our consortium project. Furthermore, we will also focus on studying the salt-tolerance mechanism of rice in this sub-project. Salinity is one of the serious abiotic stresses for plant growth, and it reduces the crop productivity. In order to produce the enough food for the continuously increasing population on the limited land, one of the most important things is to improve the salt tolerance of crops. Soil salinity caused water deficient and ion imbalance stress in cells. Some reports indicated that kinds of the defense mechanisms could be induced in plant cells to against salt stress. Although several genes involved to response the salt stress was identified, the molecular mechanisms of salt tolerance in plants are still unclear. Most of the previous studies related to the salt tolerance were focused on the model plants - Arabidopsis, and the knowledge about it in crops is limited. Effects of salt stress on crop production are board. Since it is expected to improve the quantity and quality of products in salt-stressed crops in the future, an effort will be made in our project to identify genes that are responsive to the salt stress in rice (Oryza sativa). To achieve this, we will screen the rice mutants with increased and decreased sensitivity to salt stress from the T-DNA gene trapping and activation tagging rice (TNG67) library for salt-responsive genes and promoters screening. Then, the function and regulatory mechanisms of salt stress responsive genes and promoters would be analyzed. In the future, these salt-responsive genes and promoters will be applied to increase the salt tolerance of rice plants.水稻T-DNA 突變系鹽害逆境Oryza sativa,rice,T-DNA mutant,salt-stress